OBJECTIVE To investigate the effect of slow cryopreservation on the morphology and function of primate primordial follicles within ovarian tissue slices. DESIGN Fresh monkey ovarian tissue was frozen by slow cryopreservation and thawed for analysis of morphologic and functional parameters. SETTING University-affiliated laboratory. ANIMALS Rhesus monkey ovarian tissue. INTERVENTION(S) No...

severe discharge of sewage and industrial effluents into the persian gulf leads to the deposition of various types of heavy metals, especially lead and mercury, in the muscles of fish. total mercury and methylmercury contents were determined in the edible parts (muscle tissue, fillet) of two different most popular frozen fish species from the persian gulf to ascertain whether the concentrations...

BACKGROUND The effects of exposure time (20 versus 45 s) to a high concentration of cryoprotectant (7.0 mol/l ethylene glycol with 0.5 mol/l sucrose) and freezing rates (1200-10 300 degrees C/min) during rapid freezing of mouse pronuclear stage embryos on survival and development to blastocysts were investigated. Different freezing rates were achieved by directly plunging the straws (rapid free...

background and objective: to determine the diagnostic accuracy and pitfalls of frozen section in ovarian tumors in one of the largest university affiliated gynecologic oncology centers in tehran, and determine the cause of discrepancies. materials and methods: we retrospectively analyzed the results of frozen section and permanent diagnoses of ovarian masses by reviewing the reports in the dep...

frozen section and touch imprint cytology are important diagnostic procedures for surgeons during operation and must be accurate if the patient is to receive maximum benefit. to assess the accuracy of intraoperative pathologic consultation including frozen section and touch imprint cytology during a five year period (1995- 2000), a retrospective survey of 1000 consecutive cases of frozen sectio...

This experiment wascarried out to explore the effect of use of combinations of permeating cryoprotectant and non permeating cryoprotectant on thequality of frozen-thawed Beluga (Huso huso) spermas compared to the use of permeating cryoprotectant as a control. For this work methanol (MeOH) and dimethyl sulfoxide (DMSO)are used aspermeatingcryoprotectantsand bovine serum albuminas a non permeatin...

Taking advantage of their optical transparency, we clearly observed the third stage infective juveniles (IJs) of Steinernema feltiae freezing under a cryo-stage microscope. The IJs froze when the water surrounding them froze at -2°C and below. However, they avoid inoculative freezing at -1°C, suggesting cryoprotective dehydration. Freezing was evident as a sudden darkening and cessation of IJs'...

In computer systems today, speed and responsiveness is often determined by network and storage subsystem performance. Faster, more scalable networking interfaces like Fibre Channel and Gigabit Ethernet provide the scaffolding from which higher performance computer systems implementations may be constructed, but new thinking is required about how machines interact with network-enabled storage de...

ObjectiveTo study whether endometrial epithelial podocalyxin (PCX) inhibits implantation of human embryos in vitro and patients undergoing fertilization (IVF).DesignWe have recently identified PCX as a key negative regulator receptivity. Podocalyxin is expressed all cells the nonreceptive endometrium, but selectively downregulated luminal epithelium (LE) for In current study, we first investiga...

Ultrasound technology was used to analyse the freezing behaviour of orange juice. The ultrasonic properties of orange juice, specifically velocity and attenuation coefficient, were characterised as the temperature was decreased from 20 to −50 ◦C. The results were compared with NMR free induction decay data and correlated with the amount of unfrozen water present in the frozen sample. The veloci...