In this study, we evaluated the effects of genistein supplementation of the thawing extender on frozen-thawed human semen parameters. We analyzed the effect of supplementation on sperm motility, capacitation (membrane lipid disorder), reactive oxygen species (ROS) generation, chromatin condensation and DNA damage. Using this preliminary information, it maybe possible to improve the cryopreserva...

background: despite of higher pregnancy rate after frozen embryo transfer (fet) which is accepted by the majority of researches, the safety of this method and its effect on neonatal outcome is still under debate. objective: the aim of this study was to evaluate pregnancy and neonatal outcome of fet compare to fresh cycles. materials and methods: in this study,1134 patients using fresh et and 28...

The hypothesis was tested that frozen bovine oviduct epithelial cells (BOEC) could provide a physiologic and standardized system for studying motility and acrosomal changes of fresh and frozen-thawed sperm. The BOEC were collected by lavage from cows and monolayers prepared from both fresh and frozen-thawed cells. Fresh and frozen semen processed from five bulls was extended in a modified Tyrod...

The aim of this study was to assess the effect of cooling on sperm motility before and after frozenthawed stallion semen. Fifteen ejaculates of three stallions were collected with artificial vagina. The progressive motility was determined under microscope immediately after collection, cooling (5°C for 0, 2, 7 or 24 h) before frozen-thawed and cooling (5°C for 0, 2, 7 or 24 h) after the semen wa...

BACKGROUND Embryo transfer to a developed endometrium is an important prognostic factor in frozen-thawed embryo transfer cycle outcome. Vaginal estrogen, such as Vagifem vaginal tablets and Premarin vaginal cream, is a regimen used for the patients with refractive endometria. OBJECTIVE Our objective was to compare the effects of Vagifem and Premarin on the endometrial thickness of the patient...

OBJECTIVE To describe a case of monozygotic triplet pregnancy following egg donation and the transfer of a single frozen-thawed embryo. DESIGN Case report. SETTING District general hospital and regional department of fetal medicine in northeast England. PATIENT(S) A 38-year-old woman with a 2-year history of primary infertility due to severe endometriosis and poor ovarian reserve who conc...

this study was undertaken to compare the efficiency of fresh and frozen-thawed buck semen on in vitro fertilization (ivf) of goat oocytes. cumulus oocytes complexes (cocs) were collected by aspiration of 2-6 mm diameter follicles, which were obtained from slaughterhouse. upon grading, only normal quality cocs were maturated in tcm-199 for 48 hours. the percentage of cocs reached to the m-ii sta...

BACKGROUND Cryopreservation of ovarian tissue has been suggested as an alternative to restore fertility for ovarian failure before chemotherapy. METHODS Ovaries of donor FVB/N-Tg (PolII-Luc) Ltc transgenic mice (n = 5) were cryopreserved and transplanted to the back muscles of recipient FVB/NJNarl wild-type mice that had undergone bilateral oophorectomy. We evaluated the fate of cryopreserved...

The aim of this paper was to assess whether the BMDC after freezing and thawing are capable to retain the immunophenotype and antigen-presenting capacity. BMDC were generated from bone marrow precursor cells as described previously by culturing the cells in medium containing GM-CSF and IL-4. Afterwards, the cells were harvested, counted and used for phenotyping and priming of syngeneic spleen c...