Particulate organic matter plays a significant role in the marine carbon cycle. Its sinking exports from surface to deep oceans. Using fractionated filtration, we analysed particles of 3 – 10 µm and >10 their microbiomes thirty-five stations along latitudinal transect Atlantic Ocean provide new insights into composition, community dynamics, catabolic potential particle-attached bacteria....

Campylobacter jejuni remains among the leading causes of bacterial food-borne illness. The current understanding of Campylobacter physiology suggests that it is asaccharolytic and is unable to catabolize exogenous carbohydrates. Contrary to this paradigm, we provide evidence for l-fucose utilization by C. jejuni. The fucose phenotype, shown in chemically defined medium, is strain specific and l...

Total membrane glycoproteins were labeled by growing hepatoma tissue culture (HTC) cells in the presence of [Wlfucose, and externally disposed plasma membrane glycoproteins and proteins on the same cells were labeled by reduction of oxidized galactose residues with tritiated sodium borohydride or by lactoperoxidase-catalyzed iodination. The latter labeling methods show that the plasma membrane ...

L-Fucose (6-deoxy-L-galactose) is widely distributed in nature as a component of serum glycoproteins (a), the blood group substances (3), and many plant (4) and bacterial polysaccharides (5). The pathway of biosynthesis of this compound is unknown. Dudman and Wilkinson (5) reported that Aerobacter cloacae NCTC 5920 produces a polysaccharide which contains approximately equal quantities of L-fuc...

Glycoproteins play a significant role in neoplastic transformations. Both the levels of fucose and the activity of fucosyl transferase, which mediates the assembly of the oligosaccharide moieties of the glycoprotein chains, have been found to be elevated in neoplastic conditions. Since these elevations are common features of a variety of neoplastic cells, these two have been designated as non-s...

We describe a simple, noninvasive, and effective therapy for leukocyte adhesion deficiency type II (LAD II), a rare inherited disorder of fucose metabolism. This disorder leads to an immunodeficiency caused by the absence of carbohydratebased selectin ligands on the surface of neutrophils as well as to severe psychomotor and mental retardation. The fucosylation defect in LAD II fibroblasts can ...

We describe a simple, noninvasive, and effective therapy for leukocyte adhesion deficiency type II (LAD II), a rare inherited disorder of fucose metabolism. This disorder leads to an immunodeficiency caused by the absence of carbohydrate-based selectin ligands on the surface of neutrophils as well as to severe psychomotor and mental retardation. The fucosylation defect in LAD II fibroblasts can...

Strains were constructed that contain mutational alterations affecting two distinct functional domains within the araC gene protein. The araCi (catabolite repression insensitivity) and araCh (catabolite repression hypersensitivity) mutations were used to alter the catabolite repression sensitivity domain, and mutation to D-fucose resistance was used to alter the inducer binding domain. araCh, D...

A novel exopolysaccharide named Ebosin was produced by Streptomyces sp. 139, with medicinal activity. Its biosynthesis gene cluster (ste) has been previously identified. For the functional study of the ste7 gene in Ebosin biosynthesis, it was disrupted with a double crossover via homologous recombination. The monosaccharide composition of EPS- 7m produced by the mutant strain Streptomyces sp. 1...

The isomerization of L-fucose to L-fuculose was observed by Green and Cohen (2) in extracts obtained from Escherichia coli grown on L-fucose. With the same organism and similar conditions, Huang and Miller (3) suggested that such extracts catalyzed the phosphorylation of both L-fucose and L-fuculose to form the corresponding sugar l-phosphates. The present report describes the partial purificat...