A study evaluated whether a coated (C) bacterially-derived, non-intrinsically thermostable, phytase product showed equivalent bioefficacy compared to the uncoated version of the same product (Phyzyme XP; 6-phytase, EC 3.1.3.26). Male Ross 308 chicks were assigned to three dietary treatments each with 6 cage replicates of 8 birds each The treatments were: Control (NC), NC + 500 U/kg phytase and ...

A phytase gene was identified in a publicly available metagenome derived from subsurface groundwater, which was deduced to encode for a protein of the histidine acid phosphatase (HAP) family. The nucleotide sequence of the phytase gene was chemically synthesized and cloned, in order to further overexpress the phytase in Escherichia coli. Purified protein of the recombinant phytase demonstrated ...

A meta-analysis was conducted to determine the effect of microbial phytase in pig diets on digestibility and bioavailability of Cu and Zn. Studies (n = 22) into effects of microbial phytase on digestibility and plasma levels of Cu and Zn were included in a dataset and regression analysis was performed to quantify the effect of Aspergillus niger derived 3-phytase in studies (n = 14) with a maxim...

The effects of phytate and phytase on broiler performance and cellular and humoral immunity was assessed by using 504 Cobb 500 female broilers fed nutritionally marginal diets. At 1 d of age, the chicks were randomly allocated to 6 treatments in a 2 x 3 factorial arrangement including 2 levels of phytate P (0.22 or 0.44%) and 3 dose rates of an Escherichia coli-derived phytase [0, 500, or 1,000...

A 14-d experiment was conducted to study the effects of 2 dietary variables on efficacy of a 6-phytase from Citrobacter braakii on broiler growth performance and tibia mineralization. Diets were formulated with or without nutrient matrix values for phytase as negative or positive control (NC or PC, respectively) and with 2 Ca:total P (tP; 2:1 or 2.5:1). The diets were supplemented with 0, 1,000...

conclusions the present study suggests that the enzyme obtained from strain b. subtilis can be used as feed supplement in animal diet also for reduction of phosphorus pollution problem in areas of livestock production. materials and methods the present study was conducted in biotechnology laboratory, motilal nehru national institute of technology, allahabad, uttar pradesh, india. to isolate phy...

Improving the efficiency of P use by pigs is especially important for lactating sows, whose metabolic requirements for P and Ca are high. The effect of a sp. phytase on lactating sow performance and nutrient digestibility was investigated using the combined data set for 6 studies. Treatments included a nutritionally adequate positive control diet (PC), a negative control diet (NC; with an avera...

A 14-d broiler experiment was conducted to assess the effects of 2 dietary variables on efficacy of a bacterial 6-phytase from Citobacter braakii on nutrient and phytate P (PP) utilization. Diets were formulated with or without nutrient matrix values (matrix) for phytase as negative control (NC) or positive control (PC), respectively, and with 2 Ca:total P (tP) levels (2:1 or 2.5:1). The diets ...

The Escherichia coli phytase gene appA was highly expressed in the methylotrophic yeast Pichia pastoris under the control of the AOX1 promoter. Replacement of culture medium with fresh medium in order to remove repressing glycerol and metabolic wastes prior to methanol induction significantly improved phytase expression. The phytase activity level was enhanced from 118 to 204 U/ml at the flask ...

The effect of phytase on the performance, AMEn, and the ileal digestibility of N and amino acids was investigated in a 15-d trial using day-old male broilers with diets that were low in Ca (0.9% for control and 0.79% for phytase treatment) and available P (AP; 0.45% for control and 0.35% for phytase treatment). The assayed dietary phytase activity of crumble diet was 1,149 phytase unit (FTU)/kg...