Fusarium graminearum Z-3639 and F. sporotrichioides NRRL3299 produce the trichothecene mycotoxins 15-acetyldeoxynivalenol and T-2 toxin, respectively. These toxins differ in oxygenation at C-4, C-7, and C-8. In F. sporotrichioides, Tri1 (FsTri1) controls C-8 hydroxylation. To determine the function of an apparent F. graminearum Tri1 (FgTri1) homolog, both FsTri1 and FgTri1 genes were heterologo...

Genetic diversity and identification of fumonisin-producing isolates of Fusarium verticillioides from two provinces in the Philippines were analyzed using molecular techniques. Using a Polymerase Chain Reaction (PCR)-based technique, 49 of the 54 isolates were identified as F. verticillioides, with an amplified product of 800 bp using VERT-1 and VERT-2 primers. Of these, VERTF-1/VERTF-2 primers...

In this study a total of 16 Fusarium verticillioides strains isolated from corn feed samples were characterized by fumonisin (FB) production and random amplified polymorphic DNA (RAPD). All the strains produced FB1 and FB2 with levels ranging from 2.41 to 3996.36 μg/g, and from 1.18 to 1209.91 μg/g, respectively. From the 16 F. verticillioides strains, four were identified as low (3.59 to 1289....

Levels of mycotoxins produced by Fusarium species in genetically modified (GM) and near-isogenic maize, were determined using multi-analyte, microbead-based flow immunocytometry with fluorescence detection, for the parallel quantitative determination of fumonisin B1, deoxynivalenol, zearalenone, T-2, ochratoxin A, and aflatoxin B1. Maize varieties included the genetic events MON 810 and DAS-591...

Pathogenicity of 10 Fusarium verticillioides isolates, originated from grain of wheat (five isolates) and maize (five isolates), were studied under greenhouse conditions. Based on different parameters of the pathogenicity estimate (a scale for % of nonemerged plants, % of survived plants, plant vigour — the growth and dry weight of roots and epicotyls and disease severity) it was determined tha...

Fusarium verticillioides is the primary causal agent of Fusarium ear and kernel rot in maize, producing fumonisin mycotoxins that are toxic to humans and domestic animals. Rapid detection and monitoring of fumonisin-producing fungi are pivotally important for the prevention of mycotoxins from entering into food/feed products. Chicken-derived single-chain variable fragments (scFvs) against cell ...

Real-time PCR (qPCR) is the principal technique for the quantification of pathogen biomass in host tissue, yet no generic methods exist for the determination of the limit of quantification (LOQ) and the limit of detection (LOD) in qPCR. We suggest using the Youden index in the context of the receiver operating characteristic (ROC) curve analysis for this purpose. The LOQ was defined as the amou...

ABSTRACT Fusarium ear rot and fumonisin contamination are serious problems for maize growers, particularly in the southeastern United States. The lack of maize genotypes highly resistant to infection by Fusarium verticillioides or to fumonisin contamination emphasizes the need for management strategies to prevent contamination by this mycotoxin. Information on the initial appearance of infectio...

ABBREVIATIONS: AFLP (Amplified Fragment Length Polymorphism); BAC (Bacterial Artificial Chromosome); CAPS (Cleaved Amplified Polymorphic Sequence); CPCSD (California Planting Cotton Seed Distributors); EST (Expressed Sequence Tag); FOV (Fusarium oxysporum f. sp. vasinfectum); FW (Fusarium Wilt); QTL (Quantitative Trait Locus); RAPD (Random Amplified Polymorphic DNA); RIL (Recombinant Inbred Lin...

Fusarium species are causal agents of fungal diseases occurring frequently in numerous agriculturally important plants, including potato, garlic and are one of the common pathogens of tomato, causing root rot in the west part of Iran. Therefore, the objectives of this study were to isolate and identify disease-causing Fusarium species from infected tomatoes based on the morphological and molecu...