A rat chondrosarcoma cell line and bovine cartilage explants have been used to study the control of aggrecan degradation by chondrocytes treated with interleukin-1 (IL-1) or retinoic acid (RA). Aggrecan fragment analysis with anti-neo-epitope antibodies suggests that aggrecanase (an as yet unidentified enzyme) is the only aggrecan-degrading proteinase active in these cultures. With rat cells, a...

This study investigated the diversity of rotavirus strains recovered from young children in Lagos, Nigeria, during December 1996-January 1997. In total, 287 children, aged 1-60 month(s), presenting with diarrhoea to the Gbaja Health Centre of Massey Street Children Hospital and the Lagos University Teaching Hospital, were included in the study. Rotavirus-positive specimens were characterized by...

Through a differential screening technique, we have identified a cDNA clone with differential expression in normal versus tumor cells. This clone, designated rit42 (reduced in tumor, 42 kDa), was previously isolated as a homocysteine-inducible gene in human endothelial cells (RTP), and the same or a highly related androgen-responsive gene in mouse has also been identified. Both Northern blot an...

A quantitative method using artificial antibody to detect creatine kinases was developed. Linear epitope sequences were selected based on an artificial-epitope mapping strategy. Nine different MIPs corresponding to the selected peptides were then fabricated on QCM chips. The subtle conformational changes were also recognized by these chips.

A human antibody library constructed by utilizing a phage display system was used for the isolation of human antibodies with neutralizing activity specific for human rotavirus. In the library, the Fab form of an antibody fused to truncated cp3 is expressed on the phage surface. Purified virions of strain KU (G1 serotype and P[8] genotype) were used as antigen. Twelve different clones were isola...

In renal cell carcinoma (RCC), the level of higher gangliosides is correlated with degree of metastatic potential, and cell lines derived from metastatic deposits of RCC are characterized by high expression of disialogangliosides (Saito, S., Orikasa, S., Ohyama, C., Satoh, M., and Fukushi, Y. (1991) Int. J. Cancer 49, 329-334 and Saito, S., Orikasa, S., Satoh, M., Ohyama, C., Ito, A., and Takah...

For integers n,s,b0,…,bn with n≥3,s≥0, |b0|=|bn|=1, let G1(x)=G1(x,n,s)≔n!∑j=0nbj(j!)−1n+s−jn−jxj. n≥0 and 0≤s≤92 it is proved in Shorey Sinha (2022) that, except for finitely many pairs (n,s),G1(x)=G1(x,n,s) either irreducible or linear factor times an polynomial. If s≤30, we determine here explicitly the set of (n,s) above assertion. This implies a new proof result Nair (2015) that G1(x) s≤22.

To elucidate the nature of the cross-talk between the p53 protein and the DNA repair machinery, we have investigated the relationship between the two throughout the cell cycle. Base excision repair (BER) was analyzed in cell cycle phase-enriched populations of lymphoid cells expressing wild-type p53. Our study yielded the following novel findings: (a) BER exhibited two distinct peaks of activit...

the main aim of this study was to obtain the crimean-congo hemorrhagic fever virus (cchfv) glycoprotein, through either stable transgenic plants or using a transient expression system, and determine the yield, quality and finally the immunogenicity of the plant-made cchfv glycoprotein in a mouse model. we designed and synthesized a codon-optimized g1/g2 gene from the g1 and g2 parts of the cchf...