Glucose-6-phosphate dehydrogenase (G6PDH, EC 1.1.1.49) from Leuconostoc pseudomesenteroides (the previously named: Leuconostoc mesenteroides) catalyzes the oxidation of glucose-6-phosphate in the presence of the coenzyme NADP or NAD. The enzyme is one of the industrially important enzymes and is applied in clinical settings. It is mainly useful in determinations of glucose and creatine kinase b...

A three-point linkage group comprised of loci coding for adenosine deaminase (ADA), glucose-6-phosphate dehydrogenase (G6PDH), and 6-phospho-gluconate dehydrogenase (6PGD) is described in fish of the genus Xiphophorus (Poeciliidae). The alleles at loci in this group were shown to assort independently from the alleles at three other loci--isocitrate dehydrogenase 1 and 2, and glyceraldehyde-3-ph...

In trypanosomatids, glucose-6-phosphate dehydrogenase (G6PDH), the first enzyme of the pentosephosphate pathway, is essential for the defense of the parasite against oxidative stress. Trypanosoma brucei, Trypanosoma cruzi, and Leishmania mexicana G6PDHs have been characterized. The parasites' G6PDHs contain a unique 37 amino acid long N-terminal extension that in T. cruzi seems to regulate the ...

The mechanisms of cisplatin resistance, one of the major limitations of current chemotherapy, has only partially been described. We previously demonstrated that cisplatin-resistant ovarian cancer cells (C13), are characterized by reduced mitochondrial activity and higher glucose-dependency when compared to the cisplatin-sensitive counterpart (2008). In this work we further characterized the rol...

Leptin, a protein produced and secreted by adipocytes, is know to regulate food intake and whole-body energy metabolism, but knowledge about its possible effect in bovine mammary gland is scarce. Leptin may be involved in the regulation of glucose transport even though this effect at the tissue level remains controversial. Once uptaken by the mammary gland, glucose is utilised in several ways b...

This work reports the development of an amperometric glucose-6-phosphate biosensor by coimmobilizing p-hydroxybenzoate hydroxylase (HBH) and glucose-6-phosphate dehydrogenase (G6PDH) on a screen-printed electrode. The principle of the determination scheme is as follows: G6PDH catalyzes the specific dehydrogenation of glucose-6-phosphate by consuming NADP(+). The product, NADPH, initiates the ir...

Tonoplast-enriched membranes were prepared from maize (Zea mays L. cv LG 11) primary roots, using sucrose nonlinear gradients. The functional molecular size of the tonoplast ATP-and PPi-dependent proton pumps were analyzed by radiation inactivation. Glucose-6-phosphate dehydrogenase (G6PDH) was added as an internal standard. Frozen samples (-196 degrees C) of the membranes were irradiated with ...

Background: Cryopreservation of farm animal embryos is nowadays a widely used routine technique. Success in cryopreservation of form animal depends on many factors including: freezing medium, types of cryoproctants, melting process, lipid content in ooplasm and oocyte quality It has been found that immature Oocytes with reduced reproductive performance or slaughtered at the end of their use are...

D-glucose 6-phosphate dehydrogenase (G6PDH) catalyzes the oxidation of glucose 6-phosphate (G6P) to 6-phosphogluconate-6-lactone and thereby constitutes the step which introduces hexoses into the "pentose cycle" pathway. This well-known enzyme, which is widely distributed in nature, was purified to varying degrees from different sources (for literature see refs. 1 and 2). The yeast enzyme,3 4 w...

Coccinia indica leaves were extracted with 60 % ethanol, solvents were evaporated and the residue was suspended in water. This suspension was administered orally at a dose of 200 mg/kg body wt. after 18 h of fasting to normal fed and streptozotocininduced male diabetic rats (180-250 g). After 90 min the rats were killed, and blood-glucose, hepatic glucose-6-phosphatase, fructose-1,6-bisphosphat...