human  basophils play a key role in allergic diseases such as asthma and in a variety of  immunological disorders.  the  generation  of  il-4  and  il-13  can  be  induced  from basophil  by  ige-mediated   and   non-ige-mediated   mechanisms.  time   and   stimulus- dependent  differences  in  the  regulation  of  these  cytokines  could  have  relevance  to their biological effects. the aim o...

Objective(s) Nonviral vector can be an attractive alternative to gene delivery in experimental study. In spite of some advantages in comparison with the viral vectors, there are still some limitations for efficiency of gene delivery in nonviral vectors. To determine the effective expression, the recombinant Escherichia coli lacZ genes were cloned into the different variants of pcDNA3.1 and the...

The locus of enterocyte effacement (LEE), which includes five major operons (LEE1 through LEE4 and tir), enables enterohemorrhagic Escherichia coli (EHEC) O157:H7 to produce attaching and effacing lesions on host cells. Expression of LEE2, LEE3, and tir is positively regulated by ler, a gene located in LEE1. Transcriptional regulation of the esp operon (LEE4), however, is not well defined. Tran...

besides being a metabolite of nucleotides like atp, adenosine is a mediator of neuronal function in the central nervous system. its actions are mediated by at least three extracellular receptors. in this review different aspects of adenosine such as biosynthesis, release, inactivation and its receptors are discussed. it also covers pre- and postsynaptic effects as well as postreceptor mechanism...

Marine red macroalgae have emerged to be renewable biomass for the production of chemicals and biofuels, because carbohydrates that form the major component of red macroalgae can be hydrolyzed into fermentable sugars. The main carbohydrate in red algae is agarose, and it is composed of D-galactose and 3,6-anhydro-L-galactose (AHG), which are alternately bonded by β1-4 and α1-3 linkages. In this...

the potential of bulk starter fermentation strategy for production of a cost-effective and safe source of β-galactosidase was investigated. three different bulk starter media with different compositions were selected, and an industrial yogurt starter culture strain, l. bulgaricus dsm 20081 was cultivated in these media under ph-controlled conditions (ph=5.6) at 43°c. the media consisted of 1) b...