PACKETT, L. V. (Purdue University, Lafayette, Ind.), AND R. W. MCCUNE. Determination of steam-volatile organic acids in fermentation media by gas-liquid chromatography. Appl. Microbiol. 13:22-27. 1965.-Five gas chromatographic liquid phases (25% Carbowax 20M plus 4% H3PO4, 17.5% dioctyl sebacate plus 7.5% sebacic acid, 17.5% dioctyl sebacate plus 7.5% docosanoic acid, 5% Tween 80, and 20% LAC29...

Partial derivatives of hyocholic acid were formed under the usual conditions for trifluoroacetylation of bile acids (trifluoroacetic anhydride, 35 degrees C for 20 min). Complete trifluoroacetylation of hyocholic acid was achieved at 80 degrees C for 30 min, or at 60 degrees C for 30 min when a trace of pyridine was added to the reaction mixture.

Supercritical fluid and microbore liquid chromatography offer potential applications for drug analysis. In supercritical fluid chromatography (SFC), the mobile phase is a gas (e.g., carbon dioxide) maintained at its supercritical state--that is, above its critical temperature and pressure, above which it cannot be liquefied even with further increases in applied pressure. The SFC mobile phase h...

The parameters affecting the separation and quantification of triglycerides by gas-liquid chromatography have been investigated with the use of QF-1 and SE-30 as stationary phases and a flame ionization detector. The isothermal characteristics of a wide variety of triglycerides (carbon number 6 to 60) on both columns show that long retention volume is directly proportional to carbon number and ...

Primary bile acids were studied as possible colon tumor promoters or inhibitors in a rat model of chemically induced colon cancer. Cholic acid feeding increased the number of animals with tumors, the number of tumors per animal, and the number of tumors per tumor-bearing animal. Tumor enhancement was attributed to deoxycholic acid, the bacterial metabolite of cholic acid. When chenodeoxycholic ...

1. The gas-liquid-chromatographic separations of the acetate, propionate, n-butyrate, isobutyrate and n-valerate esters of androsterone, aetiocholanolone and dehydroepiandrosterone were studied on a 1% neopentyl glycol sebacate column. The n-butyrate, isobutyrate and n-valerate esters were well resolved. 2. The three steroids derived from hydrolysed urinary 17-oxo steroid conjugate extracts wer...

An abscisic acid derivative was formed by reaction with pentafluorobenzyl bromide which allowed highly sensitive detection by gas-liquid chromatography with electron capture detection. In comparison to the methyl ester derivative, the pentafluorobenzyl derivative of abscisic acid was four times more sensitive to electron capture detection and was stable at room temperature in the presence of ul...

The cellular fatty acid composition of Brucella canis and Brucella suis was determined by gas-liquid chromatography. The presence of relatively large amounts of a 19-carbon cyclopropane fatty acid in B. suis was a major distinguishing feature between these organisms. The gas-liquid chromatography test for cellular fatty acids provides an additional criterion for the distinction of antigenically...

The triacylglyceride components of cottonseed oil were isolated and positively identified by a combination of high performance liquid chromatography (HPLC) and gas chromatography (GC). Both reversed-phase HPLC and capillary GC were capable of separating the oil into triacylglyceride peaks. These peaks were isolated by HPLC and their component acyl groups were converted to fatty acid methyl este...

The cellular fatty acid composition of Legionella longbeachae was determined by gas-liquid chromatography. As in other Legionella species, the fatty acids of this new species are characterized by relatively large amounts of branched-chain acids.