Analytical ultracentrifugation is a classical technique that has played a critical role in laying the foundations for modern molecular biology. Among its achievements is the demonstration that proteins are macromolecules rather than complexes of smaller units, and direct support for the semiconservative replication mechanism of DNA as proposed by Watson and Crick. Unlike techniques such as SDS ...

Rat lymph nodes secrete a sulphated macromolecule. This molecule was isolated by ion-exchange chromatography and partially characterized by gel-permeation chromatography, before and after chemical and enzymic modification, and electrophoresis on polyacrylamide gels, paper and glassfibre paper. The results obtained suggest that the label was not incorporated into a proteoglycan but entered a gly...

Soluble molecularly imprinted nanoparticles were synthesised via iniferter initiated polymerisation and separated by size via gel permeation chromatography. Subsequent fractionation of these particles by affinity chromatography allowed the separation of high affinity fractions from the mixture of nanoparticles. Fractions selected this way possess affinity similar to that of natural antibodies (...

Human sera were subjected to analytical ultracentrifugation and "high-pressure" gel-permeation chromatography on a system of combined TSK Gel G5000 PW and G3000 SW columns. The chromatographic method produced remarkably superior resolution of the proteins, especially those exceeding 100 000 Da. We calculated the molecular masses of eluted fractions on the basis of their detection by low-angle l...

in the present investigation efforts have been made to synthesis polyvinyl pyridine (pvpy) /c60 conjugatesthrough 2, 2-azobisisobutyronitrile (aibn) initiated insitu polymerization of pvpy in scco2.the processof synthesis was monitored under different reaction conditions ranging 1200-1800psi, 60-90 oc over 6hours. the progress of polymerization was monitored rheoviscometrically. the concentrati...