BACKGROUND A high rate of resistance to 3rd generation cephalosporins among Enterobacteriaceae isolates from Egypt has been previously reported. This study aims to characterize the resistance mechanism (s) to extended spectrum cephalosporins among resistant clinical isolates at a medical institute in Cairo, Egypt. METHODS Nonconsecutive Klebsiella pneumoniae (Kp), Enterobacter cloacae (ENT) a...

To study molecular epidemiology of CTX-M-55-carrying Escherichia coli isolates from urinary tract infections (UTIs) in China. 111 blaCTX-M-55-positive E.coli isolates from UTIs patients in China were studied. Pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) were used to analyze the homologies among the strains. Conjugation experiments, S1nuclease PFGE and PCR analys...

Sir, In Gram-negative bacteria, extended-spectrum b-lactamases (ESBLs) are widespread enzymes conferring resistance to most b-lactams, including third-generation cephalosporins but with the exception of carbapenems and cephamycins. In animals, the blaCTX-M-1 gene is one of the most frequently reported ESBL-encoding genes; in contrast, in humans the blaCTX-M-15 gene is highly prevalent. Plasmids...

OBJECTIVE To investigate CTX-M genotypes among extended-spectrum β-lactamase-producing Escherichia coli (ESBL-EC) isolated from patients with community-onset and hospital-onset infections in China, their clonality and the distribution of CTX-M variants in different specimens of community-onset and hospital-onset infections. METHODS ESBL-EC isolates were collected from general hospitals from 2...

We screened 30 Klebsiella pneumoniae isolates from dogs and cats at a single animal hospital in Guangdong Province, China. Among them, 12 K. pneumoniae strains possessed high-level resistance to amikacin and gentamicin and these were screened for 16S rRNA methyltransferase (16S-RMTase) genes. And then the genes positive isolates were detected for ESBLs (extended spectrum β-lactamases) and analy...

5 Abstract Aim: To analyse the occurrence of bla genes (blaIMP, blaVIM, blaGIM, blaSIM, blaSPM, blaPER, blaKPC, and blaOXA) responsible for carbapenemresistance, as well as the presence of cephalosporin-resistance genes (blaCTX-M, blaTEM, blaSHV and blaampC ) by polymerase chain reactions (PCRs). Methods: The study was performed on routine samples subjected to culture and antibiotics susceptibi...

infections. The spread of CTXM–positive bacteria considerably changes the way we think about treating community-acquired infections and limits the oral antibiotics that may be administered. This finding has major implications for treating children, who should not be given fluoroquinolones and tetracyclines. The observation that different blaCTX-M alleles, located on plasmids of different sizes,...

Background: There are scarce reports about the association of Klebsiella oxytoca ( K. ) with urinary tract infection (UTI) in children. We aimed to evaluate prevalence fimA, mrkA, matB and pilQ adhesins genes extended-spectrum beta-lactamase (ESBL) blaCTX-M, blaTEM blaSHV by polymerase chain reaction (PCR) study biofilm formation antibiotics resistance from children UTI. Methods: This was a ret...

background: beta-lactamase enzymes are considered the most important factor of resistance against b-lactam antibiotics among gram-negative bacteria. in recent years, the production of extended-spectrum b-lactamases has been prevailed among bacteria, especially bacteria of animal origin, and this is important in terms of public health. objective: the prupose of this study is to evaluate the pres...

The Dilúvio stream is part of an important watershed in the city of Porto Alegre, southern Brazil. It receives rain runoffs and domestic and hospital sewage, which carries a highly diverse microbial population. Some of these microorganisms may show resistance to different antimicrobials, acting as disseminators of resistance genes. This study characterizes the Gram-negative population present i...