Turnip (Brassica rapa ssp. rapa) is an important vegetable crop producing glucosinolates (GSLs) and phenolic compounds. The GSLs, phenolic compound contents and transcript levels in hairy root cultures, as well as their antioxidant, antimicrobial and anticancer activity were studied in turnip. Transgenic hairy root lines were confirmed by polymerase chain reaction (PCR) and reverse transcriptio...

Transformed hairy roots induced on Solanum dulcamara plants by Ri plasmid of Agrobacterium rhizogenes strain 8196 and A4T, exhibited a higher levels of auxin and cytokinin activity as compared with that of non transformed control roots. Endogenous pbytohormones; auxins and cytokinins were determined in an ethyl acetate fractionated extracts using bioassay methods of Hordium coleoptile test for ...

an efficient transformation system for the medicinal plant portulaca oleracea was established using agropine-type agrobacterium rhizogenes atcc15834. hairy roots were obtained directly from cotyledon leaves explants seven days after inoculation with the bacteria. the highest transformation efficiency was obtained from cotyledon leaves explants, and amounted to 53.3% within two weeks. roots grew...

To identify key genes in the regulation of salt tolerance in the mangrove plant Bruguiera gymnorhiza, transcriptome profiling in the lateral and main roots under conditions of salt stress was performed. Statistical analysis revealed that 175 and 403 of 11,997 genes shoewd significantly increased high expression in the lateral and main roots respectively. One hundred and sixty genes were up-regu...

Transformed roots are a very promising source of biologically active compounds, but industriallevel production has proved very challenging. Artemisia annua produces artemisinin, a sesquiterpene lactone which is a potent antimalarial. Transformed roots of A. annua have exhibited increased levels of artemisinin when grown in a nutrient mist bioreactor. Additionally, roots showed no evidence of ox...

Hairy root transformation has been widely adapted in plant laboratories to rapidly generate transgenic roots for biochemical and molecular analysis. We present hairy root transformations as a versatile and adaptable model system for a wide variety of undergraduate laboratory courses and research. This technique is easy, efficient, and fast making it an ideal tool for undergraduate teaching. Stu...

The aim of the study was to obtain transformed roots of Rhaponticum carthamoides and evaluate their phytochemical profile. Hairy roots were induced from leaf explants by the transformation of Agrobacterium rhizogenes strains A4 and ATCC 15834. The best response (43%) was achieved by infection with A4 strain. The effects of different liquid media (WPM, B5, SH) with full and half-strength concent...

A number of plants can be successfully transformed by Agrobacterium rhizogenes through the transfer of T-DNA from agrobacteria to the plant genome. Transgenic tissue – hairy roots – is produced as a result of the transformation process. This organized, genetically stable, hormone-independent transformed tissue is capable of accomplishing complex metabolic pathways, including biosynthesis and ac...