Fragments of glycoprotein G (gG-2(281-594His)), comprising residues 281 to 594 of herpes simplex virus type 2 (HSV-2), glycoprotein G of HSV-1 (gG-1(t26-189His)), and glycoprotein D of HSV-1 (gD-1(1-313)), were expressed in the baculovirus expression system to develop an assay for the detection of HSV-1 and HSV-2 type-specific antibodies. The expression of the gG-1(t26-189His) and gG-2(281-594H...

To gain knowledge on the precision of an in vitro method for characterisation of the fermentability of dietary fibres, this study aimed to evaluate the repeatability and reproducibility of such a method. Substrates used were citrus pectin (CP), fructo-oligosaccharides (FOS), guar gum (GG), sugar beet pulp (SBP) and wheat middlings (WM). Each substrate was incubated with faecal inoculum from fiv...

Sucrose phosphorylase (SPase) can transfer the glucosyl group of sucrose to different compounds and has been widely used in industry. To overcome low thermostability from Leuconostoc mesenteroides ATCC 12291 (LmSP), a method named PROSS was construct mutants with increased thermostability. All variants were screened by measuring their residual activities after heating at 50°C. Then, single poin...

Given a set P of n points in the plane, the order-k Gabriel graph on P , denoted by k-GG, has an edge between two points p and q if and only if the closed disk with diameter pq contains at most k points of P , excluding p and q. We study matching problems in k-GG graphs. We show that a Euclidean bottleneck perfect matching of P is contained in 10-GG, but 8-GG may not have any Euclidean bottlene...

Herpes simplex virus type 1 (HSV-1) encodes 11 envelope glycoproteins, of which glycoprotein G-1 (gG-1) induces a type-specific antibody response. Variability of the gG-1 gene among wild-type strains may be a factor of importance for a reliable serodiagnosis and typing of HSV-1 isolates. Here, we used a gG-1 type-specific monoclonal antibody (MAb) to screen for mutations in the immunodominant r...

background bipolar disorder is a biological brain disorder which is associated with debilitating fluctuation in mood and adverse effects on patients, their families and society. the importance of genetics and its role in bipolar disorder is a controversial issue to discuss. evidence indicates a relation between the risk of bipolar disorder and specific genes. amongst the genes whose role has be...

Given a set P of n points in the plane, the order-k Gabriel graph on P , denoted by k-GG, has an edge between two points p and q if and only if the closed disk with diameter pq contains at most k points of P , excluding p and q. We study matching problems in k-GG graphs. We show that a Euclidean bottleneck matching of P is contained in 10-GG, but 8-GG may not have any Euclidean bottleneck match...