نتایج جستجو برای: globin

تعداد نتایج: 6744  

Introduction: Among the factors that may be associated with the re-expression gamma-globin in adults is the methylation pattern of the promoter region. The study aimed to determine the association between promoter methylation pattern of the gamma-globin gene in the carriers and affected beta-thalassemia individuals and its expression levels. Methods: This study has been done as a case control-...

2005
Hong-yuan Luo Bryan J. Clarke David H.K. Chui

Human embryonic e globin chains were isolated from lysates of cultured human leukemic K562 cells induced with hemin. Mice were immunized with globin chains, and a murine hybridoma cell line was established that secreted monoclonal anti-c globin antibody. A sensitive and specific slot blot immunoassay for e globin chains has been developed. #{128} Globin chains were shown to be present in hemoly...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 2001
D X Feng D P Liu Y Huang L Wu T C Li M Wu X B Tang C C Liang

After screening a bacterial artificial chromosome of human genomic DNA library with human HS-40, zeta-, alpha-, and theta-globin probes, a 110-kb clone bearing the whole human alpha-globin gene cluster was obtained and rare restriction endonuclease mapping was performed. The bacterial artificial chromosome DNA was isolated, and transgenic mice were generated. Three founders were detected from 3...

Journal: :FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2014
Bradley McColl Betty R Kao Preeyachan Lourthai Kasey Chan Hady Wardan Mark Roosjen Orane Delagneau Linden J Gearing Marnie E Blewitt Saovaros Svasti Suthat Fucharoen Jim Vadolas

Expression of fetal γ-globin in adulthood ameliorates symptoms of β-hemoglobinopathies by compensating for the mutant β-globin. Reactivation of the silenced γ-globin gene is therefore of substantial clinical interest. To study the regulation of γ-globin expression, we created the GG mice, which carry an intact 183-kb human β-globin locus modified to express enhanced green fluorescent protein (e...

Journal: :Haematologica 2017
Sachith Mettananda Christopher A Fisher Jackie A Sloane-Stanley Stephen Taylor Udo Oppermann Richard J Gibbons Douglas R Higgs

Thalassemia is the world’s most common form of inherited anemia, and in economically undeveloped countries still accounts for tens of thousands of premature deaths every year. The accumulation of free excess α-globin chains in red blood cells and their precursors, as a result of the decreased production of b-globin, is believed to be the main pathophysiological mechanism leading to hemolytic an...

Journal: :Clinical chemistry 2008
Christopher Wright Donald Bergstrom Hongyue Dai Matthew Marton Mark Morris George Tokiwa Yanqun Wang Thomas Fare

BACKGROUND Blood-based biomarker discovery with gene expression profiling has been hampered by interference from endogenous, highly abundant alpha- and beta-globin transcripts. We describe a means to quantify the interference of globin transcripts on profiling and the effectiveness of globin transcript mitigation by (a) defining and characterizing globin interference, (b) reproducing globin int...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 2008
Juan C Opazo Federico G Hoffmann Jay F Storz

Phylogenetic reconstructions of the beta-globin gene family in vertebrates have revealed that developmentally regulated systems of hemoglobin synthesis have been reinvented multiple times in independent lineages. For example, the functional differentiation of embryonic and adult beta-like globin genes occurred independently in birds and mammals. In both taxa, the embryonic beta-globin gene is e...

2001
Celia Shen Che-Kun James Shen

The a globin gene locus of the common gibbon (Hylobates lar) was isolated, and it contains two closely linked a globin genes that share the same arrangement as that found for the homologous genes of other catarrhine primates. The nucleotide sequences of the gibbon a globin genes were determined and compared with the a globin gene sequences from other primate species (human, chimpanzee, oranguta...

Journal: :Haematologica 2009
Kanitta Srinoun Saovaros Svasti Worrakavee Chumworathayee Jim Vadolas Phantip Vattanaviboon Suthat Fucharoen Pranee Winichagoon

BACKGROUND beta-thalassemia occurs from the imbalanced globin chain synthesis due to the absence or inadequate beta-globin chain production. The excessive unbound alpha-globin chains precipitate in erythroid precursors and mature red blood cells leading to ineffective erythropoiesis and hemolysis. DESIGN AND METHODS In vitro globin chain synthesis in reticulocytes from different types of thal...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 1987
S Karlsson T Papayannopoulou S G Schweiger G Stamatoyannopoulos A W Nienhuis

A high-titer amphotropic retroviral vector containing the neomycin resistance gene and a hybrid gamma-beta genomic human globin gene has been constructed. Mouse erythroleukemia cells infected with this virus were found to contain the full transcriptional unit of the transferred human globin gene by Southern blot analysis. These cells contain normally initiated, spliced, and terminated human glo...

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