نتایج جستجو برای: globodera rostochiensis
تعداد نتایج: 420 فیلتر نتایج به سال:
Much progress has been made in developing techniques for characterizing population dynamics of nematodes. Nevertheless, most assay procedures have numerous inherent limitations (1,13). The efficiency of given methods may be dependent on soil type, moisture, and other environmental and biological factors (1,13). Certain extraction methods (e.g., Baermann funnel) depend on nematode motility. In a...
The potato cyst nematodes (PCN) Globodera pallida and G. rostochiensis are major pests of potatoes. The G. pallida (and G. rostochiensis) life cycle includes both diapause and quiescent stages. Nematodes in dormancy (diapause or quiescent) are adapted for long-term survival and are more resistant to nematicides. This study analysed the mechanisms underlying diapause and quiescence. The effects ...
Southern analysis showed that Gr-EXPB1, a functional expansin from the potato cyst nematode Globodera rostochiensis, is member of a multigene family, and EST data suggest expansins to be present in other plant parasitic nematodes as well. Homology modeling predicted that Gr-EXPB1 domain 1 (D1) has a flat beta-barrel structure with surface-exposed aromatic rings, whereas the 3D structure of Gr-E...
نماتد سیستی سیبزمینی،Globodera rostochiensis در سال 1387 از مزارع سیبزمینی شهرستان بهار در استان همدان گزارش شد. جهت تعیین پاتوتیپ G. rostochiensis، تعداد ده جمعیت از مزارع آلوده سیبزمینی در منطقه بهار جمعآوری شد. شناسایی گونه بر اساس خصوصیات ریختشناسی و ریختسنجی لاروهای سن دوم و سیستها و با استفاده از آغازگر اختصاصی گونه و تعیین ترادف نوکلئوتیدی ناحیه D2-D3 از ژن زیر...
Globodera infestation was evaluated in soils of private farm plots Chernihiv and Kyiv regions Ukraine 2017–2018. Soil samples were taken at 88 farms, 15.02 ha total, 11 settlements 6 districts. The nematode abundance the examined areas graded as follows: less than 1000 eggs + larvae/100 cm3 — low rate infestation; 1000–5000 average rate; more 5000 high rate. 85.4 % study area is infested with g...
Relative gene expression analyses by qRT-PCR (quantitative reverse transcription PCR) require an internal control to normalize the expression data of genes of interest and eliminate the unwanted variation introduced by sample preparation. A perfect reference gene should have a constant expression level under all the experimental conditions. However, the same few housekeeping genes selected from...
Nematodes that parasitize plant roots cause huge economic losses and have few mechanisms for control. Many parasitic nematodes infect plants by reprogramming root development to drive the formation of feeding structures. How nematodes take control of plant development is largely unknown. Here, we identify two host factors involved in the function of a receptor ligand mimic, GrCLE1, secreted by ...
The potato cyst nematode Globodera rostochiensis invades roots of host plants where it transforms cells near the vascular cylinder into a permanent feeding site. The host cell modifications are most likely induced by a complex mixture of proteins in the stylet secretions of the nematodes. Resistance to nematodes conferred by nucleotide-binding-leucine-rich repeat (NB-LRR) proteins usually resul...
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