Studies on antibody formation "in vitro" have supplied information concerning the site of antibody production (1-5), the rates of antibody synthesis per unit weight of tissue (6-7) and the percentage of active cells in lymphoid tissue during the primary and secondary antibody response (8-9). In vitro experiments have also been used in attempts to elucidate the mechanism of induction of antibody...

BACKGROUND Antithymocyte globulin or human Jurkat T-cell-line-derived antilymphocyte globulin is used in allogeneic stem cell transplantation to induce in vivo T-cell depletion to facilitate engraftment and lower graft-versus-host disease. In vitro studies suggest that antithymocyte globulin, besides causing T-cell depletion, has strong anti-myeloma activity. DESIGN AND METHODS We evaluated t...

The cellular sites of formation of gammaglobulin in lymphatic tissues of man and in a representative human lymphoid infiltrate have been studied by fluorescent antibody technique. The findings indicate that gamma-globulin is formed in the germinal centers of lymphatic nodules and in the cytoplasm of mature and immature plasma cells of two types-those with and those without Russell bodies. The g...

ONSIDERABLE TIME AND EFFORT has been devoted to the problem of fractionating serum protein simply, reliably, and rapidly, and at the same time in conformity with the free electrophoretic separation into albumin and a-, fi-, and 7-globulins. Much progress has been made, but discrepancies remain, especially in connection with aand -gIobulins. A few years ago Wolfson, Cohn, et at. (1) devised a re...

The effect of highly purified rheumatoid factor on the precipitin reactions of various antigen-antibody systems was determined. The amount of nitrogen precipitated was increased over a broad range when the factor was added to ovalbumin, human albumin, or human gamma globulin, and the corresponding rabbit antibodies. In the zone of antigen excess, soluble antigen-antibody complexes were precipit...

The problem as to whether or not the globulin fractions obtained from blood serum are chemical entities has been the subject of much discussion recently. Svedberg (l), basing his views on particle (molecular) weight determination, came to the conclusion that there is only one serum globulin, the molecular weight of which he determined to be 103,800. Sorensen (2) concluded, however, from solubil...

Previous experiments (l-3) have shown that following hypophysectomy the serum globulin concentration of dogs and rats increases and at the same time the serum albumin level falls. In the above studies serum albumins and globulins were separated by salt fractionation (4). With the aim of checking our earlier results and of obtaining more detailed information regarding the changes in serum protei...

The problem as to whether or not the globulin fractions obtained from blood serum are chemical entities has been the subject of much discussion recently. Svedberg (l), basing his views on particle (molecular) weight determination, came to the conclusion that there is only one serum globulin, the molecular weight of which he determined to be 103,800. Sorensen (2) concluded, however, from solubil...

The colloid or protein osmotic pressure (Pi) is a function of protein molarity (linear) and of Donnan and other effects. Albumin is the major osmotic protein, but also globulins influence Pi. Equations based on concentrations of albumin and nonalbumin (globulin concentration + fibrinogen concentration) protein approximate Pi better than albumin alone. Globulins have a wide range of molecular we...