The possible structural and catalytic functions of the nine tryptophan amino acid residues, including Trp(54), Trp(105), Trp(112), Trp(141), Trp(148), Trp(165), Trp(186), Trp(198), and Trp(203) in Fibrobacter succinogenes 1,3-1,4-beta-D-glucanase (Fs beta-glucanase), were characterized using site-directed mutagenesis, initial rate kinetics, fluorescence spectrometry, and structural modeling ana...

Bacillus circulans IAM1165 produces at least two extracellular beta-1,3-glucanases that lyse fungal cell walls. One of these extracellular enzymes was purified to homogeneity. The molecular mass was 87 kDa, and the pI was 4.3. The optimum temperature of the enzyme reaction was 70 degrees C when laminarin (a soluble beta-1,3-glucan) was used as the substrate. The pH range of the enzyme was broad...

Strains of the gastrointestinal species Lactobacillus reuteri were electrotransformed with plasmid constructs containing the endo-1,3-1,4-beta-glucanase gene (bglM) of Bacillus macerans. The enzyme was expressed and secreted by the lactobacilli. A plasmid construct containing the bglM gene lacking its promoter was derived and was demonstrated to be useful as a promoter probe vector.

BACKGROUND Over-expressed native or recombinant proteins are commonly used for industrial and pharmaceutical purposes, as well as for research. Proteins of interest need to be purified in sufficient quantity, quality and specific activity to justify their commercial price and eventual medical use. Proteome quality was previously positively correlated with ribosomal fidelity, but not on a single...

Powdery mildew infection in cucumber plants was significantly reduced by foliar application of 2,6dichloroisonicotinic acid (INA). Although the overall isozyme banding patterns of superoxide dismutase (SOD) and peroxidase (POD) were very similar, there were differences in the densities of major bands between the leaves of control and INA-applied plants. When plants were subjected to foliar appl...

The β-1,3-glucanases synthesized by filamentous fungi have wide applicability in the food, chemical, and pharmaceutical industries. However, its obtainment can be costly, especially due to substrates used induce synthesis. Therefore, objective of this work was produce β-1,3-glucanase T. harzianum Rifai using free immobilized cells synthetic plant sponges, different inducing that could provide b...

one hundred twenty male broiler chickens of 20 d of age were used to study the effect of enzyme supplementation on the apparent metabolisable energy corrected for nitrogen in corn, wheat and triticale using total excreta collection or marker methods. the average apparent metabolisable energy corrected for nitrogen in corn, wheat and triticale on dry matter basis, were 3480 ±90, 3263 ±61 and 326...

The effect of supplementing two commercial multienzyme to diets included two barley cultivars (Sararood [71.99%] and Valfajr [66.39%]) on performance, egg quality, and blood parameters of laying hens was investigated in an 8-wk (65 to 73 wk of age) experiment.  The commercial multienzymes were Grindazym™ (with mainly β-glucanase and xylanase activity) and Hemicell® (with mainly β-mannanase acti...

Purification of extra-cellular cellulolytic enzymes from C e l l u l o mssp. strain 0, isolated from forest soil in the north of Iran , was studied by using gel filtration and ion-exchange chromatography. Two endo-glucanases (Endo-I & Endo-II) and one exo-glucanase (Exo-I) were purified to hornogenity . The purified enzymes had molecular weights of about 39.000,70.000 and 90.000 daltons for ...

We reported earlier that ethylene increased the levels of glucanase (endo-/3-1, 3-glucan-3-glucanohydrolase, EC 3 .2. 1 .6) in bean (Phaseolus vulgaris L. cv. Red Kidney) leaves (1, 2) at Frederick, Maryland. While the function for glucanase has not been firmly established, a number of roles have been proposed and include digestion of storage glucans in seeds, control of cell elongation, regula...