Kinetic and binding studies with substrates, products, and a spin-labeled product analogue of glutathione (sl-glutathione) have been used to characterize the kinetic mechanism and properties of the catalytic site of the homodimer YaYa of glutathione S-transferase. Product inhibition studies and inhibition by sl-glutathione indicate the random addition of substrates. The kinetically determined d...

glutathione s-transferase (gst) is a superfamily enzyme which plays a major role indetoxification of xenobiotic compounds by catalyzing the conjugation of xenobiotic to cellular glutathione(gsh). gst-p is an important class of gsts which is expressed during the early stage of life and duringdevelopmental stages. its activity is relatively high during embryogenesis and immediately after birth an...

three levels of lysine (90, 100 and 110% of ross requirement) and of pyridoxine (3, 6 and 9 mg kg-1) were used in a 3 × 3 factorial experiment to investigate the growth and blood antioxidant ability of broilers under high ambient temperature. none of the dietary supplements affected the weight gain during the starter and grower periods. although no significant differences were detected between ...

this work analyzes the effects of satureja khuzestanica essential oil (skeo) on the thyroid and antioxidant system, assessed by measuring levels of tri-iodothyronine (t3), thyroxine (t4), thyroid-stimulating hormone (tsh), malondialdehyde (mda), reduced glutathione (gsh), and glutathione peroxidase (gpx) activity. forty adult male sprague dawley rats (225 ± 25 g) were divided into five equal gr...

due to the binding of the –coo- group with the mercury (ii), gold nanoparticlesfunctionalized with glutathione can self-assemble to form a supermolecular network in the hac-naac buffer solution. the aggregation of functionalized gold nanoparticles and mercury (ii)caused the shift of maximum absorption peak of the uv-vis spectrum from 520 nm to 609 nm.based on this principle, a simple spectropho...

fascioliasis is a worldwide parasitic disease in human and domestic animals. the causative agents of fascioliasis are fasciola hepatica and fasciola gigantica. in the recent years, fasciola resistance to drugs has been reported in the many of publications. fasciola spp has detoxification system including gst enzyme which may be responsible for its resistance. therefore , the aim of the study wa...

thioredoxins (trxs) are small ubiquitous oxidoreductase proteins with two redox-active cys residues in a conserved active site (wcg/ppc) that regulate numerous target proteins via thiol/disulfide exchanges in the cells of prokaryotes and eukaryotes. the isoforms ostrx23 with a typical active site (wcgpc) and ostrx20 with an atypical active site (wctpc) are two  trx h- type isoforms in rice that ...

objective(s): because of the high total phenolic contents of feijoa sellowiana , its nephroprotective effect was determined in 3,4-methylene dioxymethamphetamine (mdma) treated mice.   materials and methods: animals received 10, 20 and 40 mg/kg doses of aqueous or methanol extract of f. sellowiana leaves, intra-peritoneally. after one hour, an acute administration of mdma (20 mg/kg, ip) was inj...

background: the glutathione s-transferase (gst) family of metabolising enzymes plays an important role in the detoxification of mutagens and carcinogens. the expression of many of these cancer susceptibility enzymes is genetically polymorphic. an increased frequency of gst-null genotypes has been associated with several malignancies. objective: to investigate the rate of gstt1 and gstm1 null ge...