In many bacterial species, the glycine riboswitch is composed of two homologous ligand-binding domains (aptamers) that each bind glycine and act together to regulate the expression of glycine metabolic and transport genes. While the structure and molecular dynamics of the tandem glycine riboswitch have been the subject of numerous in vitro studies, the in vivo behavior of the riboswitch remains...

Accumulation of (14)C-labeled glycine and microelectrode techniques were employed to study glycine transport and the effect of glycine on the membrane potential (Deltapsi) in Lemna gibba G1. Evidence is presented that two processes, a passive uptake by diffusion and a carrier-mediated uptake, are involved in glycine transport into Lemna cells. At the onset of active glycine uptake the component...

The formation constants of the species formed in the systems fr + glycine + dioxovanadium(V) + and H+ + glycine have been determined in aqueous solutions of methanol at 25 °C and constant ionicstrength, using spectrophotometric and potentiometric techniques. It was shown thatdioxovanadium(V) forms two mononuclear 1 : land 1:2 complexes with glycine. The formationconstants in various media were ...

In Xenopus oocytes injected with rat brain mRNA, as in neurons, glycine greatly potentiated responses of the N-methyl-D-aspartate (NMDA) type of excitatory amino acid receptor. Injected oocytes generated a partially desensitizing inward current in response to NMDA with 30 nM added glycine. As the added glycine concentration was increased from 30 nM to 1 microM, the NMDA response was increased a...

Previously, we showed that ethanol increases synaptic glycine currents, an effect that depends on ethanol concentration and developmental age of the preparation. Glycine receptor (GlyR) subunits undergo a shift from alpha2/beta to alpha1/beta from neonate to juvenile ages, with synaptic glycine currents from neonate hypoglossal motoneurons (HMs) being less sensitive to ethanol than those from j...

Three experiments were conducted to determine whether there is a causative relation between dietary glycine concentration and intestinal Clostridium perfringens growth in broiler chickens. Expt. 1 showed that glycine concentrations were higher (P < 0.05) in jejunum and ileum of birds fed fat-encapsulated glycine compared with crystalline glycine. In Expt. 2, 2 cages of 6 birds were assigned to ...

We examined the effect of ondansetron, an antagonist of type 3 serotonin receptors, on the whole cell response of freshly isolated hippocampal CA1 pyramidal neurons of neonatal and "mature" rats to glycine using the gramicidin perforated patch technique. Ondansetron depressed the current induced by subsaturating concentrations of glycine (IGly) in a concentration-dependent manner. The ondansetr...

1. Samples of portal venous and femoral arterial plasma and mesenteric lymph were collected from unanaesthetized rats before and during the intra-duodenal infusion of high concentrations (100 mmoljl) of glycine or glycine oligopeptides (up to and including tetraglycine). 2. There was a marked increase in the concentration of free glycine, and a small amount of diglycine was detected in all the ...