Recombinant human erythropoietin (EPO) is the main therapeutic glycoprotein for treatment of anemia in cancer and kidney patients. The in-vivo activity EPO carbohydrate-dependent with number sialic acid residues regulating its circulatory half-life. carries three N-glycans thus obtaining pure glycoforms provides a major challenge. We have developed robust reproducible chemoenzymatic approach to...

he purpose of this study was to analyze the genomic information glycoprotein gene rabies virus (RABV) in several countries Asia. The genomics vaccine strain and some local isolates were retrieved from GeneBank® (National Center for Biotechnology Information, USA). Ten informations are consist ERA virus, two viruses Indonesia, a Myanmar, India, Nepal, South Korea, Japan, Sri Lanka, Iran. This bi...

Objective: Coronavirus disease-19 (COVID-19) is global pandemic which caused by SARS-CoV-2 infection. Mechanism of infection initiated attachment between viral glycoprotein with ACE2 receptor in human cells. Furthermore, Indonesia had a massive diversity plants high potency drugs, such as Pogostemon cablin Benth. In brief, it contained various volatile compounds many therapeutic properties. The...

Influenza C virus possesses specific neuraminate-O-acetylesterase as a receptor-destroying function. This enzymatic activity of the viral glycoprotein HEF (Hemagglutinin, esterase activity and fusion factor) can be visualized in situ by the use of distinct color substrates. Hereby the localization, as well as the quantity of synthesized HEF protein is detectable. We further developed the estera...

Antiphospholipid syndrome (APS) is typically characterized by increased levels of three classes antiphospholipid antibodies, namely lupus anticoagulant, anti-β2-glycoprotein I (anti-β2GPI), and anticardiolipin antibodies. β2-Glycoprotein (β2GPI) a phospholipid-binding protein composed five domains (DI–V) major antigen in APS. β2GPI expressed on the surfaces several cell types, including endothe...

background: improving vaccine potency in the induction of a strong cell-mediated cytotoxicity can enhance the efficacy of vaccines. necrotic cells and the supernatant of necrotic tumor cells are attractive adjuvants, on account of their ability to recruit antigen-presenting cells to the site of antigen synthesis as well as its ability to stimulate the maturation of dendritic cells. objective: t...

High morbidity and mortality of influenza virus infection makes it an important disease world-wide. Mouse is a very well-studied animal model for this disease with similar manifestation to human disease. It would be desirable to induce mucosal as well as circulating immune responses to obtain protection from infection and to decrease the spread of the virus. Cell mediated immunity (proliferativ...

Background: Improving vaccine potency in the induction of a strong cell-mediated cytotoxicity can enhance the efficacy of vaccines. Necrotic cells and the supernatant of necrotic tumor cells are attractive adjuvants, on account of their ability to recruit antigen-presenting cells to the site of antigen synthesis as well as its ability to stimulate the maturation of dendritic cells. Objective: T...

Soybean agglutinin (SBA) represents the anti-nutritional molecule in soybean. This agglutinates erythrocytes of several animal species. The specific binding SBA to glycoprotein c...