Four monosaccharide glycosyltransferases have been found to be present in particles prepared from normal canine respiratory tissue, and this report describes some of their properties. One is a sialyltransferase which catalyzes the transfer of sialic acid from cytidine monophosphate N-acetylneuraminic acid to glycoproteins containing N-acetylgalactosamine as terminal residues. Another transferas...

dTDP-activated sugar nucleotides are the most diverse in nature. They serve as glycosylation donors of glycosyltransferases to produce various carbohydrate structures. In their Research Article (e202217894), Liuqing Wen and co-workers have developed a general strategy for efficient preparation from readily available starting materials. This high-yielding synthetic avoids need complicated purifi...

Intact chicken embryo neural retina cells have been shown to catalyze the transfer of galactose-(14)C from uridine diphosphate galactose (UDP-galactose) to endogenous acceptors of high molecular weight as well as to exogenous acceptors. Four lines of evidence indicate that the galactosyltransferases catalyzing these reactions are at least partly located on the outside surface of the plasma memb...

The Golgi apparatus undergoes morphological changes under stress or malignant transformation, but the precise mechanisms are not known. We recently showed that non-muscle myosin IIA (NMIIA) binds to the cytoplasmic tail of Core 2 N-acetylglucosaminyltransferase mucus-type (C2GnT-M) and transports it to the endoplasmic reticulum for recycling. Here, we report that Golgi fragmentation induced by ...

Molecular characterization of plant cell wall glycosyltransferases is a critical step towards understanding the biosynthesis of the complex plant cell wall, and ultimately for efficient engineering of biofuel and agricultural crops. The majority of these enzymes have proven very difficult to obtain in the needed amount and purity for such molecular studies, and recombinant cell wall glycosyltra...

Glycosaminoglycans, a class of linear polysaccharides composed of repeating disaccharide units containing a hexosamine, are important carbohydrates found in many organisms. Vertebrates utilize glycosaminoglycans in structural, recognition, adhesion, and signaling roles. Certain pathogenic bacteria produce extracellular capsules composed of glycosaminoglycans or glycosaminoglycanlike polymers th...