presence of antibiotic resistance markers has always been considered as one of the main safety concerns in transgenic plants and their derived products. elimination of antibiotic selectable markers from transgenics is a major hurdle for finding efficient and safe candidates. herbicide tolerance genes might be attractive alternatives. in this study, a variant form of the 5-enoylpyruvyl shikimate...

EPSP synthase is the target enzyme of glyphosate herbicides. Due to extensive use glyphosate, it very important obtain EPSPS genes with high resistance for development transgenic crops. GR79-EPSPS a class I certain isolated from glyphosate-contaminated soil. After more than 1000 generations, Y40I substitution was identified, and had nearly 1.8-fold decrease in Km [PEP] 1.7-fold increase Ki[glyp...

In many crops, weeds are managed by herbicides, mainly due to the decrease in crop yields and farmers’ incomes caused by them. In general, chemical control of weeds is considered to be an easy, relatively cheap, and highly effective method. However, not all weeds can be successfully controlled, either because of their natural tolerance or their herbicide resistance. Glyphosate is one of the mos...

BACKGROUND AND AIMS With the advent of transgenic crops, genetically modified, herbicide-resistant Brassica napus has become a model system for examining the risks and potential ecological consequences of escape of transgenes from cultivation into wild compatible species. Escaped transgenic feral B. napus and hybrids with compatible weedy species have been identified outside of agriculture and ...

The 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS; EC 2.5.1.19) is a key enzyme in the shikimate pathway for the production of aromatic amino acids and chorismate-derived secondary metabolites in plants, fungi, and microorganisms. It is also the target of the broad-spectrum herbicide glyphosate. Natural glyphosate resistance is generally thought to occur within microorganisms in a strong s...

Introduction of glyphosate resistance into crops through genetic modification has revolutionized crop protection. Glyphosate is a broad-spectrum herbicide with favorable environmental characteristics and effective broad-spectrum weed control that has greatly improved crop protection efficiency. However, in less than a decade, the utility of this technology is threatened by the occurrence of gly...

Glyphosate-based herbicides (GBHs), consisting of glyphosate and formulants, are the most frequently applied herbicides worldwide. The declared active ingredient glyphosate does not only inhibit the EPSPS but is also a chelating agent that binds macro- and micronutrients, essential for many plant processes and pathogen resistance. GBH treatment may thus impede uptake and availability of macro- ...

Glyphosate is the most widely used herbicide for weed control in citrus orchards Brazil; therefore, it likely that several species have gained resistance to this and more than one resistant can be found same orchard. The objective was identify weeds glyphosate from different regions of São Paulo State (SP) determine how many are present within Seeds Amaranthus deflexus, A. hybridus, Bidens pilo...

Weed resistance to herbicides is a natural phenomenon that exerts selection on individuals in a population. In Brazil, glyphosate resistance was recently detected in Digitaria insularis. The objective of this study was to elucidate mechanisms of weed resistance in this plant, including genetic variability, allelism, amino acid substitutions, gene expression, and enzymatic activity levels. Most ...

An Eleusine indica population was previously reported as the first global case of field-evolved glufosinate resistance. This study re-examines glufosinate resistance and investigates multiple resistance to other herbicides in the population. Dose–response experiments with glufosinate showed that the resistant population is 5-fold and 14-fold resistant relative to the susceptible population, bas...