Antibody 14F3 is a murine monoclonal antibody that recognizes an antigen expressed on in vitro colony-forming cells, including virtually all CFU-GM. CFU-Meg, BFU-E, and CFUMix. In the present study we examined whether cells that do not express the 14F3 antigen include precursors of hematopoietic colony-forming cells. Colony-forming cells were depleted from marrow by treatment with l4F3 and comp...

the human granulocyte-macrophage colony stimulation factor (hgm-csf) gene was cloned in the pet 23a( +) expression vector under the control of strong bacteriophage t7 transcription and translation signals. the hgm-csf gene was transferred into e. coli strainbl21 (de3)plyss andiptg was used for induction of gm-csf gene. production of the target protein was obtained as revealed by elisa and weste...

I!. Haemopoietic growth factors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113 A. lnterleukin 3 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113 I. Cells which respon.d to interleukin 3 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ....

The biologic activity of human biosynthetic granulocyte-monocyte colony stimulating factor (GM-CSF) was investigated in serum-free culture of erythroid progenitors derived from adult peripheral blood. The morphology of erythroid bursts and the cloning efficiency of BFU-E under serum-free conditions were similar to those observed in dishes with fetal bovine serum (FBS). For these experiments, pr...

Early erythroid precursor cells and myaloid progenitor cells (CFU-GM) from four patients with 5q syndrome ware cultured in order to study the in vitro growth patterns and to determine the clonal origin of this hamatologic disorder. Cultures of CFU-GM exhibited normal colony growth. while erythroid progenitor cells demonstrated a marked decrease or absence of colony growth. Chromosomal studas in...

Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a regulatory, glycoprotein necessary for the proliferation, maturation, and survival of myeloid cells [4]. Myelopoiesis is enhanced when recombinant human (rh) GM-CSF is given to mice, monkeys [6], and some patients with various forms of neutropenia [3, 5]. The clinical use of rhGM-CSF infusions is under intensive investigation now. A...

Recombinant rat stem cell factor (rrSCF) and recombinant human granulocyte colony-stimulating factor (G-CSF) coinjected for 1 week in rats cause a synergistic increase in mature marrow neutrophils accompanied by a striking decrease in erythroid and lymphoid marrow elements. The spleens of the same rats show increased granulopoiesis as well as increased erythropoiesis as compared with the spleen...

Previous studies suggest that malignant cells from some patients with myeloid leukemias produce colony-stimulating factors (CSFs) that can function as autocrine growth factors in vitro. We have examined the roles of interleukin-6 (IL-6) and granulocyte-macrophage CSF (GM-CSF) in the proliferation of myeloid leukemia cells. IL-6 activity was assessed in conditioned medium (CM) from myeloid leuke...

The effects of transforming growth factor flu or (TGF-/11 or -p2) on the in vitro proliferation and differentiation of normal and malignant human hematopoietic cells were studied. Both forms of TGF-fl suppressed both the normal cellular proliferation and colony formation induced by recombinant human interleukin-3 (IL-3) and granulocyte-macrophage colony-stimulating factor (GM-CSF). In the prese...

Purpose Growing evidence suggests that granulocyte-macrophage colony-stimulating factor (GM-CSF) contributes to T helper 17 (Th17) cell-associated immunoinflammatory diseases. The purpose of this study was to evaluate the effect of T cell-derived GM-CSF on CD11b+ myeloid cell function in dry eye disease (DED). Methods In a murine model of DED, quantitative real-time PCR and ELISA were used to...