Escherichia coli is a Gram negative facultative anerobe which rod shaped and found in the gut of warm blooded animals. Since this bacteria showed varying morphology different environmental conditions, an experimental study was conducted to find out changes culture media. E.coli seen as typical, uniformly stained long gram rods only when it cultured on MacConkey agar

The rapid 3M Prompt inoculation system was compared with the traditional log-phase system for Autoscan identification and antibiotic susceptibility testing (MIC) of 188 recent clinical isolates. The two systems were equally effective for gram-negative rods; the Prompt system was slightly superior for the determination of MICs for gram-positive organisms.