The signal-mediated and spatially controlled assembly and dynamics of actin are crucial for maintaining shape, motility, and tip growth of eukaryotic cells. We report that a novel Armadillo repeat protein in Arabidopsis thaliana, ARMADILLO REPEAT ONLY1 (ARO1), is of fundamental importance for polar growth and F-actin organization in tip-growing pollen tubes. ARO1 is specifically expressed in th...

A new three-stage computer artificial neural network model of the tip-of-the-tongue phenomenon is shortly described, and its stochastic nature was demonstrated. A way to calculate strength and appearance probability of tip-of-the-tongue states, neural network mechanism of feeling-of-knowing phenomenon are proposed. The model synthesizes memory, psycholinguistic, and metamemory approaches, bridg...

The signal-mediated and spatially controlled assembly and dynamics of actin are crucial for maintaining shape, motility, and tip growth of eukaryotic cells. We report that a novel Armadillo repeat protein in Arabidopsis thaliana, ARMADILLO REPEAT ONLY1 (ARO1), is of fundamental importance for polar growth and F-actin organization in tip-growing pollen tubes. ARO1 is specifically expressed in th...

Characteristic features of morphogenesis in filamentous fungi are sustained polar growth at tips of hyphae and frequent initiation of novel growth sites (branches) along the extending hyphae. We have begun to study regulation of this process on the molecular level by using the model fungus Ashbya gossypii. We found that the A. gossypii Ras-like GTPase Rsr1p/Bud1p localizes to the tip region and...

Mound formation in the cellular slime mould Dictyostelium results from the chemotactic aggregation of competent cells. Periodic cAMP signals propagate as multiarmed spiral waves and coordinate the movement of the cells. In the late aggregate stage the cells differentiate into prespore and several prestalk cell types. Prestalk cells sort out chemotactically to form the tip, which then controls a...

Cytoplasmic dynein is a minus-end-directed microtubule motor that participates in multiple cellular activities such as organelle transport and mitotic spindle assembly [1]. To study the dynamic behavior of cytoplasmic dynein in the filamentous fungus Aspergillus nidulans, we replaced the gene for the cytoplasmic dynein heavy chain, nudA, with a gene encoding a green fluorescent protein (GFP)-ta...

In the framework of this master thesis, a numerical study has been carried out in order to investigate the effect of tip geometry on the tip leakage flow and heat transfer features in a unshrouded high pressure axial flow turbine stage designed by Erhard [10]. Four different tip profiles were considered, namely a default flat tip, a modified flat tip, a modified double squealer tip and a modifi...

Cryosurgery is a technique for destroying undesirable tissue, such as cancers, by exposing it to a very low temperature. This paper presents a thermodynamic modeling tool that has been developed for a two stage, mixed gas Joule-Thomson (MGJT) cryoprobe used for cryosurgery. A conventional vapor compression (VC) cycle using a pure refrigerant precools the MGJT cycle providing refrigeration at th...

We demonstrate a scattering-type scanning near-field optical microscope (s-SNOM) with broadband THz illumination. A cantilevered W tip is used in tapping AFM mode. The direct scattering spectrum is obtained and optimized by asynchronous optical sampling (ASOPS), while near-field scattering is observed by using a space-domain delay stage and harmonic demodulation of the detector signal. True nea...