Modern software architecting increasingly often takes place in geographically distributed contexts involving teams of professionals and customers with different backgrounds and roles. So far, attention and effort have been mainly dedicated to individuals sharing already formalized knowledge and less to social, informal collaboration. Furthermore, in Web 2.0 contexts, little to no attention has ...

Defne Saatci, MD; Tom A. Ranger, PhD; Cesar Garriga, Ash Kieran Clift, Francesco Zaccardi, Pui San Tan, Martina Patone, Carol Coupland, Anthony Harnden, Simon J. Griffin, Kamlesh Khunti, Hajira Dambha-Miller, Julia Hippisley-Cox, MD

We recently introduced a method (Griffin, B. A.; Adams, S. R.; Tsien, R. Y. Science 1998, 281, 269-272 and Griffin, B. A.; Adams, S. R.; Jones, J.; Tsien, R. Y. Methods Enzymol. 2000, 327, 565-578) for site-specific fluorescent labeling of recombinant proteins in living cells. The sequence Cys-Cys-Xaa-Xaa-Cys-Cys, where Xaa is an noncysteine amino acid, is genetically fused to or inserted withi...

We describe a novel system, GRIFFIN (G-protein and Receptor Interaction Feature Finding INstrument), that predicts G-protein coupled receptor (GPCR) and G-protein coupling selectivity based on a support vector machine (SVM) and a hidden Markov model (HMM) with high sensitivity and specificity. Based on our assumption that whole structural segments of ligands, GPCRs and G-proteins are essential ...

W H Allum, S M Griffin, A Watson, D Colin-Jones on behalf of the Association of Upper Gastrointestinal Surgeons of Great Britain and Ireland, the British Society of Gastroenterology, and the British Association of Surgical Oncology . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ....