Physiological changes that result in changes in bacterial gene expression are often accompanied by changes in the growth rate for fast adapting enteric bacteria. Because the availability of RNA polymerase (RNAP) in cells depends on the growth rate, transcriptional control involves not only the regulation of promoters, but also depends on the available (or free) RNAP concentration, which is diff...

Ribosomal RNA (rRNA) transcription is regulated primarily at the level of initiation from rRNA promoters. The unusual kinetic properties of these promoters result in their specific regulation by two small molecule signals, ppGpp and the initiating NTP, that bind to RNA polymerase (RNAP) at all promoters. We show here that DksA, a protein previously unsuspected as a transcription factor, is abso...

Hybrid promoter constructs were used to determine the DNA sequence requirements for stringent and growth rate control within a promoter region. The promoters were obtained by fusing complementing sequence regions located upstream and downstream from the GCGC discriminator motif of the growth rate regulated rRNA P1 promoter and a non-regulated tac promoter variant. The activities and the regulat...

A 2 × 3 factorial experiment was conducted to investigate the effect of enzyme Endofeed W (EEW) at 0 and 0.05%, thyme essential oil (TEO), probiotic Primalac® (PP) and non-feed additive, on intestinal histomorphology of broilers fed wheat-based diet. Each of the 6 dietary treatments was replicated 4 times with 12 chicks each from 0 to 42 days of age. There was no interaction between enzyme and ...

Abstract The experiment was conducted in the nursery of Diyala Agriculture Directorate during growing seasons 2021 from March to November study effect growth promoters Humic acid, Atonic and Salicylic acid spray on some chemical vegetative parameters Local bloody orange transplants budded sour rootstock. included two factors, first one cultivars (Local bloody), second promoters(Humic acid). spr...