T HE IN VIVO method of tissue culture using cellulose ester filter diffusion chambers developed by Algire and co-workers1 combined with the autoradiographic method of observing cells labeled with the specific DNA precursor, tritiated thymidine, has been used in the present experiments to study lymphocytopoiesis in an isolated population of lymphoid cells. In addition, data are presented concern...

In the experiments to be described, it was observed that the administration of 7,12-dimethylbenz(a)anthracene (7,12-DMBA) prior to the injection of tritiumlabeled thymidine (TdRH3) considerably depresses the incorporation of tritium into rat testis, intestine, and adrenal. Recovery from the effect of 7,12-DMBA occurs in intestine within 48 hr but in testis the inhibitory action of a single dose...

This study was designed to answer the question: Is H3-thymidine uptake by nuclei of the mouse seminal vesicle evidence for DNA synthesis and mitosis, or does it signify some "metabolic" function of DNA unrelated to chromosome duplication? Mice were given an intraperitoneal injection of H3-thymidine. Six hours later Feulgen squashes of the seminal vesicle epithelium were made and covered with au...

Objective: Zerumbone (ZER) is a well-known natural compound that has been reported to have anti-cancer effect. Thus, this study investigated the ZER potential inhibit Thymidine Phosphorylase (TP) and ability trigger Reactive oxygen species (ROS)-mediated cytotoxicity in non-small cell lung cancer, NCI-H460, line. Material Method: The antiangiogenic activity for was evaluated by using thymidine ...

background: filamentous hemagglutinin (fha) is one of the most important immunoprotective antigens of bordetella pertussis (b.pertussis) and a major component of the acellular pertussis vaccine. in the present study, three overlapping recombinant fragments from the immunodominant region of fha were produced and their immunogenicity was investigated. methods: three overlapping coding sequences o...

Transcriptional silencing of tumor suppressor genes by aberrant DNA methylation is a characteristic frequently observed in cancer cells. Therefore, reversing this process is a therapeutic target against cancer. In this study, we established a screening system for silencing inhibitors with cell lines transfected by a retroviral vector containing a luciferase gene. More than 100 nucleosides were ...

The presence of toxic substances which inhibit erythropoiesis in uremic sera is still undetermined. We examined the effect of aceton-methylalcohol extracts of serum of nephrectomized rabbits with uremia on the erythropoiesis. The percentage red cell iron utilization was markedly reduced in nephrecto mized rabbits with uremia. The administration of aceton-methylalcohol extracts of uremic rabbit ...

One of the most enigmat ic results of studies on the enzymic synthesis of D N A is tha t the polymerase is consistently found in homogena te fractions generally designated as cytoplasmic 0 4 ) . Since an unequivocal answer to the question of intracellular location of the enzyme seemed quite impossible from homogenate studies, we have taken a direct cytological approach to the problem. T he expe...