BACKGROUND Nematode secreted haemoglobins have unusually high affinity for oxygen and possess nitric oxide deoxygenase, and catalase activity thought to be important in protection against host immune responses to infection. In this study, we generated a monoclonal antibody (48Eg) against haemoglobin of the nematode Anisakis pegreffii, and aimed to characterize cross-reactivity of 4E8g against h...

Glycated haemoglobin (HbA1c) measured by high performance liquid chromatography (HPLC) in a 20 year old female with insulin dependent diabetes mellitus was consistently within the normal range although her daily blood glucose values were > 11.1 mmol/l. HbA1c measured by immunoagglutination and fructosamine was elevated and correlated with the patient's blood glucose values. The HPLC chromatogra...

An experiment was conducted on eight weak and anaemic goats revealed pale conjunctivae, increased heart rate, rough hair coat, dullness and depression. The haematology showed significantly decreased haemoglobin (7.37±0.16 g %), packed cell volume (PCV)(23.37±0.56%),total erythrocyte count (TEC)(8.12±0.18 million/cumm), mean corpuscular haemoglobin (MCH) (9.10±0.30 pg) and mean corpuscular volum...

A method is described for the estimation of serum haptoglobin as ;haemoglobin-binding capacity'. The method relies on column chromatographic separation of the haemoglobin/haptoglobin complex from excess added free haemoglobin on the dextran gel Sephadex G.100. The method is simple and reproducible, and correlates well with another method of estimating haemoglobin-binding capacity over a wide ra...

Background Total haemoglobin measurement (tHb) is one of the most commonly performed laboratory tests in patients admitted to the neonatal intensive care unit (NICU). Non invasive haemoglobin measurement is possible. In neonates this method can reduce iatrogenic blood loss. Studies performed in adults to compare haemoglobin (Hb) obtained with the use of non-invasive Hb monitor and laboratory me...

INTRODUCTION Implantation of a ventricular assist device (VAD) is a seminal therapeutic option for patients with terminal cardiac failure. However, haemolysis remains a clinically relevant adverse effect of several VAD types. Lysed erythrocytes release lactate dehydrogenase (LDH) and haemoglobin. Free haemoglobin in plasma is bound by haptoglobin with high affinity. The complex is internalised ...