Genetic variability of the ovine parasite Haemonchus contortus from the Alpine area was investigated using mitochondrial DNA (nd4 gene), internal transcribed spacers 1 and 2 and microsatellites, in order to assess whether cross-transmission between domestic and wild ruminants occurs. The dataset was composed of 78 individual adult male H. contortus collected from chamois (Rupicapra r. rupicapra...

Aim: To study the prevalence of gastrointestinal parasites in sheep and goats of Mathura region. Materials and Methods: A total of 240 faecal samples collected from three different farms were examined by direct smear, Willi's floatation and sedimentation techniques. Quantitative examination was done by McMaster's technique. Results: Out of 240 samples processed 165 samples were found positive f...

Water was evaporated from infective Haemonchus contortus larvae suspended in tap, distilled and triple-distilled water, and the nematodes were then exposed to 50% and 75% relative humidity (RH) at 20, 30, 40, and 50 C. Sample groups were rehydrated 4 hr daily in similar quality water, observed for motility, then returned to the same RH and temperature and re-evaporated. This was continued until...

The effects of tannins on adult populations of Haemonchus contortus, Trichostrongylus colubriformis and Teladorsagia circumcincta in goats are characterised mainly by a decrease in egg excretion without any significant changes in worm number. In contrast, the impact of tannins on T. colubriformis or T. circumcincta third-stage larvae (L3) is associated with a significant reduction in worm estab...

A strain of Haemonchus contortus from the Pietermaritzburg district of Natal was found to be resistant to levamisole (geometric mean efficacy 76.5%), morantel (41.9%), the benzimidazoles (oxfendazole: 33.7%) and rafoxanide (82.0%), but apparently fully susceptible to closantel and disophenol. In the case of ivermectin, a mean of 5.2% of the H. contortus was not removed at a dosage of 200 microg...

excretory/secretory antigen was prepared by culturing live adult worms of haemonchus contortus in rpmi 1640 medium at a concentration of 50 worms per ml in a culture-flask at 37 ˚c for 24 hr and the culture supernatant was used as antigen. the e/s antigen was purified by thiol-sepharose affinity chromatography. on western blot analysis, it was demonstrated that thiol-purified antigen showed a s...

Four groups of 6-7-month-old Merino lambs were each dosed with 40 000 infective larvae of Trichostrongylus axei on 2 November 1976 and subsequently exposed to challenge with Haemonchus contortus on natural grazing at the University of Pretoria's Experimental Farm in the eastern suburbs of Pretoria. One of these groups and one group of controls were killed every 8 weeks from the end of December ...

Infective larvae (L3) of two different batches of anthelmintic-susceptible Haemonchus contortus, cryopreserved for 15,6 and 15,7 years in liquid nitrogen appeared dead when examined 10-20 min after thawing. However, having become motile later in the day, their viability was tested the next day in two separate groups of sheep. The mean development of 41,8% and 66,3% of the H. contortus compared ...

The objective of this study was to assess antagonism of nematophagous fungi and species producers metabolites and their effectiveness on Haemonchus contortus infective larvae (L3). Assay A assesses the synergistic, additive, or antagonistic effect on the production of spores of fungal isolates of the species Duddingtonia flagrans, Clonostachys rosea, Trichoderma esau, and Arthrobotrys musiformi...