it is an increased interest in the therapeutic potential of cannabis sativa l. (marijuana) for treatment of multiple sclerosis and hiv neuropathy. because of limitation in cultivation of this plant, an efficient hairy root induction system for cannabis sativa l. was developed in the present study. agrobacterium rhizogenes mediated transformation performed by two different co-cultivation mediums...

background & aim:oxidative stresses caused by free radicals are known to promote many diseases such as inflammation and cancer; therefore, any plant product that can inhibit free radical production can play an important role in preventing diseases. we aimed to compare the fracture rate and the scavenging activity of free radicals, and the antioxidant composition of rolb-transformed hairy root o...

The roots and rhizomes of Valeriana (Valerianaofficinalis L.) are rich with valuable metabolites such as valerenic acid and valepotriates are used as mild sedatives. The aim of the present study was to investigatethe effect of four levels of calcium and potassium compounds (half, full, 2 and 4-fold) of normal Murashige and Skoog (MS) media including KI and CaCl2on ValerianaofficinalisL. hairy r...

Following in vitro infection with Agrobacterium rhizogenes wild strain (mannopine, 8196) and two A. tumefaciens transconjugant strains (C58C1 pArA4abc and C58C1 pArA4b) transformed (hairy) roots were induced in pumpkin (C. pepo L.) cotyledons. The presence of pRi T-DNA in pumpkin long-term hairy root cultures was determined by Southern hybridization. The influence of plant growth regulators on ...

Agrobacterium rhizogenes (pRi), a causative agent of hairy root disease, effectively induces hairy root formation in a variety of plant species. In our study four bacterial strains AR15834, A4, 9435 and C318 and three explants types leaf, stems and roots, were examined. Hairy roots were induced from roots, stems and leaf explants. The highest transformation efficiency of 77% was achieved by usi...

This study evaluated the induction of hairy roots in Perovskia abrotanoides Karel, a medicinally important member of Lamiaceae family, using different strains of Agrobacterium rhizogenes and various carbon supplies, for the first time. This research established an efficient Agrobacterium mediated transformation system for this plant species through bacterial strains including ATCC15834, TR105 a...

Induced hairy roots by Agrobacterium rhizogenes are suitable organs for the production of secondary metabolites, due to the stability and high production of roots without phyhormones in a short time. Fenugreek (Trigonella foenum– graecum L.) is one of the most important medicinal plants with valuable medicinal compounds. For optimizing the hairy root induction of T. foenum-graecum L., six strai...

Hyoscyamus albus hairy roots with/without an exogenous gene (11 clones) were established by inoculation of Agrobacterium rhizogenes. All clones cultured under iron-deficient condition secreted riboflavin from the root tips into the culture medium and the productivity depended on the number and size of root tips among the clones. A decline of pH was observed before riboflavin production and root...

Plants are the important sources of drugs. Secondary metabolites are responsible for therapeutic propertiesin plants. Three compounds including (-)-hyoscyamine, its racemate atropine, and scopolamine (hyoscine) are the most famous tropane alkaloids in the Solanaceae familly. Nowadays, attempts to developthese alkaloids in biotechnological procedures which are principally based on the hairy root...

Background: Biotechnologic methods are common for secondary metabolites production from the plants and other sources in pharmaceutical sciences. Hairy root cell lines as the biotechnologic method have been used for in vitro production of major plant metabolites. Objective: In this study, hairy roots of Hypericum Perforatum have been prepared using the seeds and bio transformed by bacteria. Fin...