X-ray sensitive Chinese hamster V79 cells mutants, V-C4, V-E5 and V-G8, show an abnormal response to X-ray-induced DNA damage. Like ataxia telangiectasia (AT) cells, they display increased cell killing, chromosomal instability and a diminished inhibition of DNA synthesis following ionizing radiation. To localize the defective hamster gene (XRCC8) on the human genome, human chromosomes were intr...

The adhesive glycoprotein fibronectin has been isolated from fresh hamster plasma by affinity chromatography on gelatin coupled to Sepharose beads by the method of Engvall & Ruoslahti [Int. J. Cancer (1979) 20, 1-5]. Polyacrylamide-gel electrophoresis of material heated in sodium dodecyl sulphate and 2-mercaptoethanol shows two prominent polypeptide subunits of approx. mol.wts. 215 000 and 200 ...

SUMMARY Hamsters inoculated with murine sarcoma virus (Moloney) of mouse or rat origin developed nonregressing, metastasizing, undifferentiating sarcomas. Light microscopy and ultrastructural studies of the hamster sarcoma revealed less tumor-cell differentiation than reported for the mouse or rat. Cell-free extracts prepared from the hamster tumors were oncogenic only in animals of the same sp...

The ribosomal RNA (rRNA) of a series of hamster-mouse somatic cell hybrids was studied. Mouse 28S rRNA was separated from its hamster counterpart by a two-step procedure involving sucrose gradient centrifugation of ribosomes and polyacrylamide gel electrophoresis of rRNA. Both hamster and mouse types of rRNA were synthesized in the 11 hybrids tested, including hybrids containing only about one-...

Altered queuine modification of tRNA has been correlated to neoplastic transformation, but no direct cause and effect relationship has been defined. In the present study, a potential role for this alteration has been assigned. The tRNA in normal Chinese hamster embryo cells is significantly more queuine modified than the tRNA in their transformed Chinese hamster embryo counterparts, even though...

Complement-fixing (CF) antibody-positive sera from hamsters bearing adenovirus type 12 (Ad. 12)-induced tumors revealed specific immunofluorescent stainable antigens in essentially all Ad. 12 hamster tumor cells. The antigens were primarily in the form of cytoplasmic flecks; less frequent staining was seen as nuclear flecks or homogeneous staining of nucleus and cytoplasm of a small proportion ...

Antibodies were produced against 220,000-molecular-weight proteins of Syrian hamster embryo cells. The antiserum containing these antibodies is capable of immunologically staining the surfaces and a fibrillar network around untransformed Syrian hamster embryo cells. The staining is removed by adsorption of the antiserum with Syrian hamster embryo cells and by mild trypsin treatment. Several lin...

The growth characteristics of an oncogenic simian adenovirus, SV20, in rhesus monkey (RhMK) and hamster kidney (HamK) cell cultures were compared. The ad sorption rate of SV20 in both cell systems was rather slow, but increased steadily up to 6 hr. There was 3 to 8 times more cell-associated virus in RhMK cells than in HamK cells during the first 6 hr of infection. A complete growth cycle of SV...

In this study, we present the identification and characterization of hamster and guinea pig nicotinic acid receptors. The hamster receptor shares approximately 80-90% identity with the nucleotide and amino acid sequences of human, mouse, and rat receptors. The guinea pig receptor shares 76-80% identity with the nucleotide and amino acid sequences of these other species. [(3)H]nicotinic acid bin...

Mouse and hamster spermatozoa from the cauda epididymidis were deposited into the female tract at various periods following ligation of the corpus epididymidis. It was found that the number of spermatozoa in the cauda epididymidis decreased sooner in the hamster than in the mouse but the initial decrease in fertilizing ability occurred much earlier in the mouse than in the hamster. The fertiliz...