Intracytoplasmic sperm injection is the newest and the most successful micromanipulation technique for treating male factor infertility. It entails the mechanical insertion of a chosen spermatozoon directly in to the cytoplasm of an oocyte. The technique was used in veterinary science for the purpose of clarifying the different steps of fertilization by investigating the fusion and penetration ...

The clinical potential for fertilization was examined by using the human sperm-hamster oocyte assay system after microinjection of round (RS), elongating (ES) or elongated (EtedS) spermatids retrieved from obstructive and non-obstructive azoospermic patients. Freshly isolated, in-vitro cultured and cryopreserved spermatids were utilized. For each category of microinjected spermatids, we demonst...

INTRODUCTION It is widely recognized that all that fathers essentially contribute to the next generation is half of their genome, and mature sperm cells are transcriptionally and translationally inactive. However, an increasing number of reports have shown that RNAs were present in human sperm detected by using RT-PCR, differential display, in situ hybridization and microarrays [1]. One study s...

the objective of the current study was to determine the anatomical features of the auditory ossicles inhamster. twenty temporal bones from 10 adult hamsters, weighing 275-300 g were used. after dissectingthem, the features of the ossicles were assessed with an ocular micrometer and photographed by astereomicroscope. finally, all data were evaluated and analysed using the sigma statt software. t...

In mammals, the oocyte and its surrounding cumulus cells constitute on oocyte cumulus complex (OCC). During ovulation, OCCs are extruded into the peritoneal or bursal cavity, depending on the species, and are then rapidly picked up by the fimbria on the outer surface of the oviductal infundibulum and transported to the ampulla, where fertilization occurs. We developed a method to measure OCC pi...

introduction: in this study we examined the effect of ampullary and isthmic epithelial cell of golden hamster oviduct on n-mary mouse embryo development during 4-days in vitro co - culture, and the effect of injected gonadotropins on the quality of co-culture. materials and methods: golden hamster oviducts were isolated 18-24h after treating the hamster with hcg. they were flushed with dmem / h...

ABSTRACT Hamster breeding is becoming increasingly popular, mainly because of the simplicity breeding, low cost, and characteristics these animals: they are small, quiet, do not require a large space. Because this ease keeping, some people choose to keep more than one animal same species in single enclosure. In cases, sociability can lead fights, hamsters extremely territorial animals, which ca...

The localization and activity of mitochondria in hamster oocytes during maturation and fertilization were monitored by using three different fluorescent probes: rhodamine 123 (Rh123), MitoTracker Red (MTRed) and 3 ,3 ’ -d ihexy loxacarbocyan ine iod ide (DiOC6(3)). Oocytes aged in vivo were also examined. The germinal vesicle (GV) oocytes exhibited a range of mitochondrial organizations, from a...

the objective of this study was to investigate fungal flora of the hair coat of domestic golden hamsterswith and without skin lesions. sixty hamsters were examined in the small animal teaching hospitalof the faculty of veterinary medicine, ferdowsi university of mashhad, and mashhad, iran. asteriletoothbrush was used for sample collection, from the entire body of the hamsters, all skin lesions ...