The stability of four hemoglobins (Hb) in dimer forms (low concentration) were investigated by the kinetics of denaturation. The rate constants of denaturation were obtained by variation of 280 nm absorption versus time in 10 mM Tris-HCl, 10 mM EDTA, pH 8.0 at 45 degrees C in the absence and presence of 0.5 M ethanol, dimethyl sulfoxide (DMSO), formamide, and glycerol. The results show the tren...

Previous studies have shown that heparin-binding epidermal growth factor (EGF)-like growth factor (HB-EGF) mRNA is synthesized in the mouse uterine luminal epithelium temporally, just prior to implantation, and spatially, only at the site of blastocyst apposition (Das, S. K., Wang, X. N., Paria, B. C., Damm, D., Abraham, J. A., Klagsbrun, M., Andrews, G. K. and Dey, S. K. (1994) Development 120...

Cell-free hemoglobin (Hb) is a purified preparation of human hemoglobin that is being developed as a resuscitation fluid. In vivo administration of hemoglobin has resulted in significant toxicity, due in part to contamination with bacterial endotoxin (lipopolysaccharide (LPS)). To better understand this toxicity, we have studied the interaction between Hb and LPS. Mixtures of each of three diff...

HB and HB+ are a shared secret-key authentication protocols designed for low-cost devices such as RFID tags. HB+ was proposed by Juels and Weis at Crypto 2005. The security of the protocols relies on the learning parity with noise (LPN) problem, which was proven to be NP-hard. The best known attack on LPN by Levieil and Fouque [13] requires subexponential number of samples and sub-exponential n...

The human leukemia K562 cell line can be induced by 20 micro M hemin to reversibly accumulate embryonic and fetal hemoglobins without any change in the rate of cell division. When we reduced the rate of cell division by glutamine starvation or addition of hydroxyurea, the cells increased by tenfold the basal hemoglobin level of 0.3-0.5 pg Hb/cell. The combined effects of hemin and inhibitors of...

Parallaxes from the Hipparcos satellite allow for the first time to calibrate parameters of horizontal-branch (HB) like stars based on geometric distances. We present absolute magnitudes and luminosities for 8 such stars. Using Teff and log g values available from the literature, we also can calculate the mass of the HB stars. We find an average value ofMHB = 0.38 ±0.07 M for stars with 7500< T...

Roberts, P. M. M., Arrowsmith, D. E., Lloyd, A. V. C., and Monk-Jones, M. E. (1972). Archives of Disease in Childhood, 47, 631. Effect of folic acid treatment on premature infants. After finding that a group of premature infants became extremely deficient in folate after 3 months of age, a comparable group of premature infants was treated with 100 ,g folic acid given orally daily starting at 28...

The binding of inositol hexaphosphate (IHP) to oxyhemoglobin (oxy-Hb) was investigated by gel filtration and stopped flow kinetic methods. The stoichiometry of the complex is 1 IHP per hemoglobin tetramer in 0.05 M Z,Z-bis(hydroxymethyl)-2,2’,2”nitrilotriethanol (pH 7.0) containing 0.11 M NaCl and appears to approach 2 IHP per tetramer when the ionic strength is reduced to 0.01. The dissociatio...

References 1. Weatherall DJ. The thalassemias. In: Stamatoyannopoulos G, Majerus PW, Perlmutter RM, Varmus H, eds. The molecular basis of blood diseases, Vol. 3. Philadelphia: WB Saunders, 2001:183–226. 2. Chui DH, Fucharoen S, Chan V. Hemoglobin H disease: not necessarily a benign disorder. Blood 2003;101:791–800. 3. Lemmens-Zygulska M, Eigel A, Helbig B, Sanguansermsri T, Horst J, Flatz G. Pr...

The in vitro effect of S-stage-specific drugs on the fetal hemoglobin (Hb F) potential of erythroid precursors and progenitors was tested by exposing bone marrow cells to 5-aza-2'-deoxycytidine, Ara-C, or hydroxyurea in suspension cultures and reculturing the cells in drug-free clonal cultures. Analysis of Hb F in the erythroblasts present at the end of suspension cultures and in the erythroid ...