AIM to assess the accuracy and safety of a ready-to-use atopy patch test (HDM-Diallertest, DBV Technologies, Paris) in the diagnosis of sensitization to house-dust mite (HDM) allergens in children with or without atopic dermatitis. PATIENTS AND METHODS prospective analysis of a systematic allergic work-up was carried out in 47 children, age 57.4+42 months (mean + SD, range 7 to 176 mo), prese...

House dust mite (HDM) represents one of the most common sources of aeroallergens worldwide and more than 50% of allergic patients are sensitized to these allergenic molecules. HDM allergy research in the past has been mainly focused on adaptive, mite allergen-dependent immune responses. In recent years it has become clear that, although the allergenspecific CD4+ Th2 cells orchestrate HDM allerg...

Background The basophil activation test (BAT) is a promising tool for monitoring allergen-specific immunotherapy responses. Objective We aimed to investigate the changes in basophil activation in response to the inhalant allergens of house dust mite (HDM) and mugwort pollen during immunotherapy in patients with allergic rhinitis. Methods We enrolled patients with allergic rhinitis who were ...

House dust mite (HDM) challenge is commonly used in murine models of allergic asthma for preclinical pathophysiological studies. However, few studies define objective readouts or biomarkers in this model. In this study we characterized immune responses and defined molecular markers that are specifically altered after HDM challenge. In this murine model, we used repeated HDM challenge for two we...

WINNING ABSTRACT: Prior evidence has suggested a direct relationship between the level of house dust mite (HDM) allergen exposure, sensitisation and asthma. A recent cross-sectional study indicated a bell-shaped relationship between HDM exposure and sensitisation [1]. We examined the effects of cumulative HDM allergen exposure during the first 5 years of life on outcomes at age 5 years in Sydne...

Methods Potential T-cell epitopes were identified by algorithm, screened for ability to stimulate T-cell responses in exvivo blood samples from HDM allergic subjects and tested to confirm they did not cause basophil histamine release. A second group of HDM allergic subjects attended a challenge where Conjunctival Provocation Test (CPT) response and Early (EPSR) and Late Phase Skin Response (LPS...

BACKGROUND   Specific immunotherapy is the only treatment with the potential to prevent progression of the allergic disease and the potential to cure patients. The immunomodulatory ability of SQ-standardized house dust mite (HDM) subcutaneous immunotherapy (SCIT) was investigated in patients with allergic asthma. METHODS Fifty-four adults with HDM-allergic asthma were randomized 1:1 to receiv...

Our laboratory established a role for poly(ADP-ribose)polymerase (PARP) in asthma. To increase the clinical significance of our studies, it is imperative to demonstrate that PARP is actually activated in human asthma, to examine whether a PARP inhibitor approved for human testing such as olaparib blocks already-established chronic asthma traits in response to house dust mite (HDM), a true human...

Aeroallergens such as house dust mite (HDM), cockroach, and grass or tree pollen are innocuous substances that can induce allergic sensitization upon inhalation. The serine proteases present in these allergens are thought to activate the protease-activated receptor (PAR)-2, on the airway epithelium, thereby potentially inducing allergic sensitization at the expense of inhalation tolerance. We h...