Screening of bead-based peptide libraries against fluorescent dye-labeled target proteins was found to be significantly influenced by the dye characteristics. Commercially available red fluorescent dyes with net negative charges adversely showed strong interactions with library beads. The introduction of zwitterionic dyes significantly reduced the unwanted interactions, which sheds light upon u...

Contents 1 Introduction 1 1.1 Protein-protein-interaction screenings in vivo 2 1.1.1 Yeast two-hybrid screens 2 1.1.2 in vivo pulldown assays 4 1.1.3 Data integration and critical evaluation 6 1.2 Protein-protein-interaction screenings in vitro 6 1.2.1 Protein arrays 6 1.2.1.1 Protein microarrays 7 1.2.1.2 Arrayed cDNA expression libraries 9 1.2.2 Peptide arrays 10 1.3 Other in vitro techniques...

The zebrafish is a vertebrate model compatible with the paradigms of drug discovery. The small size and transparency of zebrafish embryos make them amenable for the automation necessary in high-throughput screenings. We have developed an automated high-throughput platform for in vivo chemical screenings on zebrafish embryos that includes automated methods for embryo dispensation, compound deliv...

In the last years high-throughput pharmacophore screenings have been rediscovered as an effective and rapid tool for guiding the selection of new hit compounds with predefined biological activity. This renaissance has also been fostered by the current possibility to generate pharmacophore hypotheses directly from crystallographic, NMR or computational models of proteinligand complexes [1,2]. In...

Background Gamelan, a traditional music ensemble has become the most popular played by Balinese people for decades, both as leisure and religious activities. Musical activity can enhance cognitive function. However, there are limited activities retired musicians. Aim Alzheimer Indonesia Nederland Foundation (ALZI Ned) initiated “Melody Memory Project” which aimed to increase quality of life dem...

Abstract: Between 1992 and 2009, 30 US states adopted laws mandating that health insurance plans cover screenings for prostate cancer. Because prostate cancer screenings are used almost exclusively by men over age 50, these mandates raise the cost of insuring older men relative to other groups. This paper uses a triple-difference empirical strategy to take advantage of this quasi-random natural...

Human skin equivalents (HSEs) can be considered a valuable tool to study aspects of human skin, including the skin barrier, or to perform chemical or toxicological screenings. HSEs are three-dimensional skin models that are usually established using primary keratinocytes and closely mimic human skin. The use of primary keratinocytes has several drawbacks, including a limited in vitro life span ...

Small or shrivelled wheat kernels (screenings) that reduce crop value are commonly produced in terminal drought environments. The aim of this study was to establish whether the incorporation of the tiller inhibition (tin) gene would contribute to maintenance of kernel weight and reductions in screenings under terminal water deficit. Five Silverstar near-isogenic lines contrasting in high and lo...

OBJECTIVE To determine the associations between personality subscales and attendance at gastric cancer screenings in Japan. METHODS A total of 21,911 residents in rural Japan who completed a short form of the Eysenck Personality Questionnaire-Revised (EPQ-R) and a questionnaire on various health habits including the number of gastric cancer screenings attended were included in the primary ana...