BACKGROUND Enteric fever is endemic in all parts of India. The Widal agglutination test is widely used for its diagnosis. But the interpretation of Widal test depends upon the baseline titre which is prevalent amongst healthy individuals in a particular geographical area. AIM Present study was undertaken to establish the normal baseline titre in apparently healthy blood donors and also to det...

From Mycobacterium tuberculosis, a serologically specific polysaccharide was purified. It contained mannose, arabinose, and a trace of glucose. It was not active in hemagglutination (HA) reaction. Partial acylation of the polysaccharide was carried out with palmitoylchloride, and the synthetic lipopolysaccharide contained 15.1 per cent of esterified palmitic acid. The synthetic lipopolysacchari...

The aim of this study was to investigate the hemagglutination properties of caprine herpesvirus-1(CpHV-1) and their application for CpHV-1 antigen detection and evaluation of the dynamics of postinfectious/vaccine antibody production. CpHV-1 hemagglutination activity was proved only with mouseerythrocytes, PBS and saline solutions at 4°C and 22°C. In vaccinated animals, goats and bucks withresp...

The interaction of lipid vesicles (liposomes) of several different compositions with erythrocytes has been investigated. Lecithin liposomes, rendered positively charged with stearylamine, exhibit potent hemagglutination activity in media containing low concentrations of electrolytes. The hemagglutination titer is found to be a linear function of the zeta potential of the lipid vesicles. Hemaggl...

Three cohort studies in adults were performed during the period from 1986 to 1989. Eight hundred and eighty-four subjects were, one or more times, immunized with influenza vaccines, and pre- and post-vaccination antibody titres were determined by hemagglutination inhibition tests. One thousand and one hundred and nineteen vaccination events in 681 subjects could be analysed by a comparison, per...

A hemagglutination method employing tanned human O erythrocytes sensitized with purified Group A streptococcal polysaccharide has been developed to measure A polysaccharide antibody in antistreptococcal rabbit and human sera. The reaction of sensitized RBC with known Group A streptococcal rabbit antisera, inhibition of hemagglutination with A polysaccharide, and abolition of hemagglutination an...

A reverse hemagglutination assay was used to study adherence to human erythrocytes by Escherichia coli H10407, which possesses colonization factor antigen I. Pretreatment of erythrocytes with trypsin, chymotrypsin, papain, protease, and neuraminidase completely abolished attachment reactivity. In addition, the hemagglutination reaction was prevented by the presence of urea and guanidine. In con...