Both length of extra-alveolar time and type of storage media are significant factors that can affect the long-term prognosis for replanted teeth. Numerous studies have examined various media in an attempt to determine the ideal material for storage of the avulsed tooth. The purpose of this study was to compare the number of viable periodontium ligament (PDL) cells in different storage media usi...

Knowledge of the exact number of viable cells in a given volume of a cell suspension is required for many routine tissue culture manipulations, such as plating cells for immunocytochemistry or for cell transfections. This protocol describes a straightforward and fast method for differentiating between live and dead cells and quantifying the cell concentration and total cell number using a hemac...

Five methods of assessing blood in urine were studied and compared: sedimentation count, hemacytometry, "Clini-lab" reagent strip and "N-Multistix" and "ChemStrip-8" dipsticks. The minimum sensitivity of two commercially available urine dip-stick procedures for blood was established. The study includes method association, assay variation among and within technologists, accuracy, precision, and ...

This method is adapted from a protocol described by Grimsley et al. (1977). For more information about P. patens as a model organism, see The Moss Physcomitrella patens: A Novel Model System for Plant Development and Genomic Studies (Cove et al. 2009a). For details about the growth of P. patens on cellophane overlay plates, see Culturing the Moss Physcomitrella patens (Cove et al. 2009b). For p...

Low-level laser therapy (LLLT) has been reported to improve tissue healing and might therefore be useful in dental pulp capping after trauma. We evaluated the effects of a low-level diode laser (lambda = 680 nm) and dental pulp-capping substances on cell proliferation. Calcium hydroxide and adhesive resin were applied as conditioned media to cultures. Half of the samples received irradiation wi...

The viability of Paracoccidioides brasiliensis yeast-form cells was determined by colony-forming units, direct fluorescent staining, and production of germ tubes in slide culture. The first procedure was unreliable and time consuming; the latter two showed better correlation with hemacytometer total cell counts and required significantly less time.

This paper presents a study of low-ultrasonic energy that can be affected to increase yeast growth. Forces of the ultrasonic stimulation in the yeast media broth, a liquid nutrient medium for the culture of yeast, produce change of liquid densities and surrounding pressure, which affect to the yeast growth. A utilized ultrasonic system including transducer of 20 kHz was varied powers and applie...

Glass fiber reinforcement of the hybrid acrylic resin with difference method can enhance residual monomer content of the material; it can cause cytotoxic effect on fibroblast cells. The purpose of this study was to know the cytotoxicity of hybrid acrylic resins after glass fiber reinforcement with difference method on the cultured fibroblasts. The squared specimens of 10 mm in length, 10 mm in ...

The present study was conducted to investigate the relationship between Mycoplasma pneumoniae (MP) infection and the risk of asthma among children by detecting the rate of MP immunoglobulin M (MP-IgM) and the eosinophil (EOS) count. A total of 139 asthmatic children were enrolled as the case group and assigned into three groups: Group A (aged <3 years, n=42), group B (aged 3-8 years, n=45) and ...

Nitrification is one of the important microbiological transformations of nitrogen in the ocean. Traditional enrichment-culture methods for enumerating the autotrophic bacteria which oxidize ammonium to nitrite are very time consuming (months) and are believed to seriously underestimate natural abundances. A fluorescent-antibody assay for a marine ammonium-oxidizing bacterium was developed to pr...