background escherichia coli o157:h7 is an enteric pathogen which can be frequently found asymptomatically in ruminant mammals, but can cause diseases from mild diarrhea to hemolytic uremic syndrome in humans. methods we developed fluorescent amplification-based specific hybridization (flash–pcr) assay to detect the stx-encoding gene stx-1 of e. coli o157:h7. result pcr product of 336 bp was suc...

Streptococcus agalactiae, also known as group B Streptococcus, is an aetiological agent of urinary tract infection (UTI) in adults, including cystitis, pyelonephritis and asymptomatic bacteriuria (ABU). Whereas ABU-causing S. agalactiae (ABSA) have been shown to grow achieve higher culture denstity human urine compared uropathogenic (UPSA) other phenotypic distinctions between isolated from dif...

Biosurfactant are surface active compounds (SAC), amphiphilic in nature consisting of hydrophobic (long chain fatty acids) and hydrophilic domain (amino acid, protein, carbohydrate). Classification this compound is based on molecular weight, mode action physico-chemical structure. being utilized laundry formulation, house hold cleaning products, as antimicrobial agents, detergents, pesticides, ...

The aim of this study was to investigate the probiotic characteristics profile isolated lactic acid bacteria (LAB) from cow milk enzymatic clots. Some criteria for potential such as fermentation profile, and bile tolerance, salt hydrolysis, antimicrobial activities, bacteriocin production assay, acidification coagulation, hemolytic properties were examined. 16S rDNA-based method applied confirm...

Fresh leaves of Erigeron floribundus were obtained, and its ethanolic extract was assessed for antimicrobial, antioxidant-free radical scavenging activity as well phytochemical constituents. Hemolytic activities also screened to know whether the will lyse blood agar. Six pathogenic bacteria; gram-positive, Bacillus cereus, Clostridium species, Staphylococcus aureus, gram-negative: Klebsiella pn...

We report double-determinant sandwich enzyme-linked immunosorbent assay measurements (4) of lipopolysaccharide (LPS) binding protein (LBP) in children with uncomplicated Escherichia coli O157:H7 hemorrhagic colitis (HC) and those with O157:H7-associated hemolytic uremic syndrome (HUS). Characteristics of these children have been previously reported (5). The analytical sensitivity of LBP measure...

Abstract The structural integrity of the host red blood cell (RBC) is crucial for propagation Plasmodium spp . during disease-causing stage malaria infection. To assess stability vivax -infected reticulocytes, we developed a flow cytometry-based assay to measure osmotic within characteristically heterogeneous reticulocyte and P. samples. We find that erythroid decreases erythropoiesis maturatio...

Diagnostic assays for measurement of functional mannose-binding lectin (MBL) in serum are widely performed as part of immune status assessment. Classical pathway mediated complement activity can interfere in these functional MBL assays. Here we describe classical pathway interference incidentally occurring in a previously described hemolytic MBL assay and the modification of this assay to preve...