the polyacrylamide gel were visualized by silver staining (Bio-Rad). PCR products were purified by poly-acrylamide gel electrophoresis and used for sequence analyses. The sequencing reactions were carried out using the Taq Dye-Deoxy Termination Cycle Sequencing Kit and DNA Sequencer (Model 373) from Applied Biosys-tems. The mutation screening showed heteroduplex formation only for exon 2. These...

Heteroduplex DNA molecules which contain both the wild-type and mutant sequences of a deletion nonhomology possess a characteristic electrophoretic mobility in agarose and can be readily separated from both the wild-type and deletion-containing parental homoduplex fragments. Because of the partial single stranded character of these deletion-containing heteroduplex molecules, they are selectivel...

interferon gamma (ifn-g) is a cytokine produced by activated t lymphocytes and natural killer cells. it plays an important role in innate and adaptive immunity. ifn-g gene is polymorphic and its haplotype has been associated with resistance to nematode infestations which are the most important parasites of domestic sheep worldwide. to evaluate ifn-g polymorphism in iranian shaul sheep populatio...

BACKGROUND The presence of a dominant T-cell clone is an important diagnostic criterion in cutaneous T-cell lymphomas (CTCLs) and in atypical T-cell cutaneous infiltrates. Procedures based on polymerase chain reaction (PCR) are the most sensitive to detect clonality, but heteroduplex analysis is less sensitive than other methods such as denaturing gradient gel electrophoresis. OBJECTIVE To as...

conclusions more studies are needed to investigate the relationship between other parts of this gene with hearing loss in different populations through the country. more research could clarify the role of this gene and its relation with deafness and provide essential information for the prevention and management of auditory disorders caused by genetic factors in the iranian population. backgrou...

Heteroduplex joints are general intermediates of homologous genetic recombination in DNA genomes. A heteroduplex joint is formed between a single-stranded region (or tail), derived from a cleaved parental double-stranded DNA, and homologous regions in another parental double-stranded DNA, in a reaction mediated by the RecA/Rad51-family of proteins. In this reaction, a RecA/Rad51-family protein ...

Heteroduplex experiments between the plasmid R6 and one strand of the deoxyribonucleic acid (DNA) of a lambda phage carrying the insertion sequence IS1 show that IS1 occurs on R6 at the two previously mapped junctions of resistance transfer factor (RTF) DNA with R-determinant DNA. From previous heteroduplex experiments, it then follows that IS1 occurs at the same junctions in R6-5, R100-1, and ...