Cyclodextrins (CDs) have long been used to manipulate cellular cholesterol levels both in vitro and in vivo, but their direct effects at a cellular level are not well characterized. Recently, CDs have garnered much interest because of their ability to clear stored cholesterol from Niemann Pick Type C (NPC) cells and markedly prolong the life of NPC1 disease mice. Here, we investigate the hypoth...

Hemoglobin (Hb) adducts of 4-hydroxy-1-(3-pyridyl)-1-butanone (HPB), a metabolite of two tobacco-specific nitrosamines: 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) and N’nitrosonornicotine (NNN), were measured in blood samples collected from 47 non-smokers, including 21 cases of lung cancer, enrolled in four centers. Exposure to environmental tobacco smoke (ETS) during the previous yea...

The paradigm introduced by William Halsted for surgical residency training has been considered the most appropriate educational system to acquire the knowledge and surgical skills required to become a competent general surgeon. Hepato-pancreato-biliary (HPB) surgery is considered an important part of general surgery training because of its high prevalence and complexity. Nowadays, we are faced ...

12-O-Tetradecanoylphorbol-13-acetate (TPA), a potent tumor promoter, induced phenotypic differentiation in the human thymic acute lymphocytic leukemia cell line, HPB-ALL. Within 30 min of seeding in the presence of TPA, the cells formed a smooth round shape. After a 7-day exposure to TPA, most of the cells became smaller and reminiscent of large or atypical lymphocytes. Electron microscopic ana...

To help determine CD83 function, a cDNA encoding a soluble protein containing the CD83 extracellular domain was fused with a mutated human IgG1 constant region (CD83Ig) and expressed by stable transfection of Chinese hamster ovary cells. Purified CD83Ig bound to peripheral blood monocytes and a subset of activated CD3CD8 lymphocytes but did not bind to FcR. Monocytes that had adhered to plastic...

1. Early-weaned lambs were used to estimate the concentration of urea required to give the maximum intake and utilization of maize or barley with either a high (HPB) or low (LPB) protein content. 2. Approximately the same concentration of urea (7--11 g urea/kg feed) was required for maximum intake and feed utilization of both HPB and LPB. With maize there was no increase in intake, live weight ...

BACKGROUND Improved imaging methods and surgical techniques have created a new era in hepatopancreatobiliary (HPB) surgery. Despite these developments, visual inspection, palpation, and intraoperative ultrasound remain the most utilized tools during surgery today. This is problematic, though, especially in laparoscopic HPB surgery, where palpation is not possible. Optical imaging using near-inf...

BACKGROUNDS/AIMS Major hepato-pancreato-biliary (HPB) surgery is usually performed via an open method rather than a laparoscopic method. Postoperative ileus (POI) is a classic complication after open surgery. The purpose of this study was to determine whether polylactic film is useful in the prevention of POI. METHODS A total of 179 patients who underwent major HPB surgery between 2005 and 20...

Endotoxin has been shown to stimulate ACTH and cortisol secretion through an action at the hypothalamic level. However, the nature of hypothalamic neurohormones, corticotropin-releasing hormone (CRH) and especially arginine vasopressin (AVP), involved in that regulation is still controversial. The purpose of this study was to determine the effects of an acute i.v. endotoxin administration on CR...

A novel multi-mode probe consisting of a hexaphenyl-1,3-butadiene derivative, 2,2'-((((1Z,3Z)-1,2,3,4-tetraphenylbuta-1,3-diene-1,4-diyl)bis(4,1-phenylene))bis(methanylylidene))dimalononitrile (ZZ-HPB-CN), with typical aggregation-enhanced emission (AEE) features was easily prepared for the highly sensitive and rapid detection of amine vapors. The ZZ-HPB-CN sensor, which was prepared by simply ...