Contact of wear particles with body fluids can result in widespread dissemination of extractable constituents from joint implants. The aim of this in vitro study is to clarify whether there is a mutagenic and/or carcinogenic risk from CoCrMo and Ti6Al wear particles. Particles of a representative size were produced by fretting; toxicity and mutagenicity were investigated using the salmonella/mi...

The cell line HepG2 is derived from a well differentiated human hepatoblastoma, which retains many of the morphological characteristics of liver parenchymal cells. These cells at passages greater than 95 were found to metabolically activate carcinogens to genotoxic metabolites. The addition of 6.8 microM 1-methyl-3-nitro-1-nitrosoguanidine, 5.3 microM 4-nitroquinoline-N-oxide, and 4-20.3 microM...

Mammals experience some degree of hypoxia during their lifetime. In response to hypoxic challenge, mammalian cells orchestrate specific responses at transcriptional and posttranslational level which lead changes in the purine metabolites order cope with threatening conditions. The aim this study was evaluate enzymes involved metabolism human muscle Muscle culture were exposed enzymatic activity...

-Hybridoma technology is reviewed, and the characteristics of monoclonal antibodies are compared with those of polyclonal antibodies. The contribution of monoclonal antibodies to molluscan pathology is developed with special emphasis on their use as diagnostic tools. The results of studies with monoclonal antibodies prepared against the protozoan oyster pathogen Bonamia ostreae are briefly desc...

LABORATORY DATA biochemical markers metabolic defect chromosomal assignment chromosome X localization chromosome Xq localization chromosome Xq27 localization chromosomal disorders chromosomal fragility, instability, breakage chromosome breakage syndromes foetal-amniotic biochemical data hypoxanthine-guanine phpsphoribosyltransferase defect (foetal) gene, structural-functional anomalies gene ana...

The structurally modified polyene antibiotic nystatin methyl ester (NME) has been utilized as a half-selection agent for isolating interspecific mouse--Syrian hamster hybrids. By using HAT media supplmented with NME we have isolated hybrid clones from polyethylene glycol-fused cultures of biochemically defective mouse (A9 or B82) and genetically normal Syrian hamster (KHK/C13) cells. Unfused pa...

Deficiency of hypoxanthine-guanine phosphoribosyltransferase (HGPRT) is a rare inborn error of purine metabolism and is characterized by uric acid overproduction along with a variety of neurological manifestations that depend on a degree of the enzymatic deficiency. Inheritance of HPRT deficiency is X-linked recessive; thus, males are generally more affected and heterozygous females are carrier...