Reverse transcriptase (RT) of Moloney murine leukemia virus (MMLV) is the most widely used enzyme for cDNA synthesis and RNA amplification. In this study, we aimed to produce MMLV RT recombinantly due its importance in molecular studies. context, DNA fragment encoding was cloned into pTOLT plasmid expressed E. coli BL21 (DE3) pLysE cells. Since high-level expression protein caused molecules agg...

This paper focuses on an application of conjoint analysis within forestry economics. More specifically, it was used to determine eucalypt plant value perceptions among South African nurserymen, to assess the financial drivers of the eucalypt production system, and to attempt to quantify the trade-offs between attributes of a eucalypt nursery and the selling price of the plants. Utility function...

Accurately predicting the required spatial resource for aggregate block assembly in shipyard production is difficult due to the following reasons: 1) lack of optimal block on-jig configuration and 2) the dynamic and stochastic nature of production system. Consequently, managerial objectives are seldom achieved without effective production planning and control. In this study, a look-ahead schedu...

MGSyn is a programming toolbox based on game-theoretic notions of synthesis for generating production code in the domain of industrial automation. Instead of painstakingly engineering sequences of relatively low-level program code, the designer selects pre-defined hardware components together with behavioral interfaces from a given library, specifies a topology for the interconnection of compon...

The paper presents an approach for detecting vehicles in urban traffic scenes by means of rule-based reasoning on visual data. The strength of the approach is its formal separation between the low-level image processing modules (used for extracting visual data under various illumination conditions) and the high-level module, which provides a general-purpose knowledge-based framework for trackin...

This position paper provides a general description of the field of active databases, focusing on the main problems yet to be solved; suggests that deductive databases may contribute to understand some of these problems; and indicates classes of applications that can be specified in a declarative way. Premise The field of active databases has recently emerged as one of the most important directi...

RELIABLE SHOT DETECTION. A shot is commonly defined as the uninterrupted recording of an event or locale. Any video sequence in this world consists of one or more shots concatenated by some kind of transition effects. Therefore, shots are generally considered as the elementary units constituting a video. Detecting shot boundaries thus means recovering those elementary video units, which in turn...