This paper describes the development and validation of analytical methodology for the determination of the use of MDMA, MDEA and MDA in urine. After a simple liquid extraction, the analyses were carried out on a high performance liquid chromatography (HPLC) in an octadecyl column, with fluorescence detection. The mobile phase using a sodium dodecyl sulfate ion-pairing reagent allows good separa...

urinary 1-hydroxypuren (1-ohp) is commonly used as a major metabolite and biological indicator of the overall exposure to polycyclic aromatic hydrocarbons (pahs). for evaluation of human exposure to such compounds, biological monitoring is an essential process, in which, preparation of samples is one of the most time-consuming and error-prone aspects prior to chromatographic techniques. in this...

KEYWORD: bisphenol A; immune-affinity column; high-performance liquid chromatography ABSTRACT: A rapid and sensitive method using immuno-affinity columns (IACs) as sample purification procedure and high-performance liquid chromatography (HPLC) tandem fluorescence detector (FLD) for detection has been developed for the determination of bisphenol A (BPA) in beer. The limit of detection (LOD) is 0...

a rapid, simple and sensitive high-performance liquid chromatography method was developed for determination of ciprofloxacin in plasma by means of ultraviolet detection. ofloxacin was used as an internal standard and separation carried on a novapak c18 column using a mobile phase of 0.01 m phosphate buffer (ph =2.6): methanol (82: 18 v/v). extraction of drug was performed from plasma by liquid-...

High-performance liquid chromatography (HPLC) with UV, circular dichroism (CD) and intrinsic fluorescence detection was applied to monitor conformational properties of recombinant human interferon alpha2b when performing size exclusion chromatography (SEC) and reversed-phase HPLC (RP-HPLC). In this way native conditions during SEC and structural changes of the protein during RP-HPLC were demons...