background diazinon is one of the most widely used organophosphrous pesticides in the world may affect animals and human. objectives this study was done to assess whether pseudomonas plecoglossicida can grow on diazinon as the sole carbon metabolic source in laboratory and agricultural soil. materials and methods serial dilutions of pure and commercial diazinon in minimal salt medium were made ...

Two different analytical methods for the determination and confirmation of ochratoxin A (OTA) in blood serum, kidney and liver of pigs have been compared. Sample clean-up was based on liquid-liquid phase extraction. The detection of OTA was accomplished with high-performance liquid chromatography (HPLC) combined either with fluorescence detection (FL) or electro spray ionization (ESI+) tandem m...

High-performance liquid chromatography (HPLC) is a refinement of traditional column chromatographic techniques. The speed of analysis and the resolution are increased with new column-packing materials and eluant pumped through the column at high pressure. The potential for achieving measurements of hormones in small volumes of plasma or urine is limited, both in normal and pathological situatio...

Over the years, separation resolution and reduction of analysis time has continually improved in High Performance Liquid Chromatography (HPLC). For further improvement, column efficiency must be increased. The relationship among separation efficiency, the mobile phase linear velocity and particle size was investigated in detail in the early 1970 s [1]. This and other systematic investigations h...

background aflatoxins are one of the most potent toxic substances that occur naturally. nowadays extensive attention has been taken to their existence in food and environment, as there is the possibility of harm to humans following chronic exposure to extremely low levels via food chain. aflatoxin m1 (afm1) is a hepatic carcinogenic metabolite found in the milk of lactating animals fed with con...

Forage-based nutrients constitute the main forage value of grass. Elymus nutans Griseb possesses a wide ecological adaptability, enhanced crude protein content, good palatability, and excellent genes. Herein, employing medium- (MPLC) high-pressure liquid chromatography (HPLC), along with online HPLC-DPPH (OHD)-based identification, two primary radical scavenging compounds were extracted identif...

A three-step procedure has been developed for the separation of complex mixtures of sterol intermediates in cholesterol biosynthesis. The method has been applied to the separation of sterol intermediates formed from [14C]mevalonate by normal rat hepatocyte culture cells. In Step 1, a short gravity-flow silicic acid column (1.2 x 6.5 cm) separates the incubation products into four classes consis...

Background: Jamu is commonly known as an Indonesian traditional herbal medicine that contains ingredients or derived from plants, animals, minerals, galenic, mixtures of these have been hereditary for medicinal use. Some studies reported the presence chemicals are deliberately added to Jamu. Jamuthat containing usually had a faster healing effect compared do not contain chemicals. cause serious...

A method for estimation of volume of alkyl bonded phases for high performance liquid chromatography (HPLC) is presented. It is based on application of macromolecular probes, which are able to elute from the HPLC column without entering the bonded groups and thus to monitor their volume. The appropriate mathematical expression of results were looked for and it was concluded that the optimum repr...