background: the hbv-x (hbx) protein is believed to contribute to the development of hcc. however, the molecular mechanisms involved in hbx-mediated hepatocarcinogenesis remain obscure. in this study, the effect of hepatitis b virus x gene and its protein product hbxag on expression of p53 gene in hep g2 cell line was investigated. methods: viral dna extracted from hbv-positive serum and hbx gen...

Investigação fitoquímica das folhas da Hosta longipes identificou um novo flavonóide glicosídeo, o caempferol-3-O-β-D-glucopiranosil-(1→2)-[6’’’-O-acetil-β-D-glucopiranósido]7-O-β-D-glucopiranósido, e mais cinco derivados flavonóides conhecidos. As estruturas de dois compostos foram reveladas por vários métodos de RMN (H e C RMN, H-H COSY, HMQC HMBC) e hidrólise química. Dados de RMN de um dele...

Three new species of Phyllosticta, P. hostae on Hosta plantaginea (China), P. schimae on Schima superba (China), and P. ilicis-aquifolii on Ilex aquifolium (UK), are described and illustrated in this study. They are compared with morphologically similar and phylogenetically closely related species. A polyphasic approach using phylogeny, host association, disease symptoms, colony and morphologic...

background and objectives: virosomes are virus like particles (vlp) assembled in-vitro. influenza virosomes maintain the cell binding and membrane fusion activity of the wild type virus but are devoid of viral genetic material or internal proteins. influenza virosomes mimic the natural antigen presentation route of the influenza virus. materials and methods: virosomes were prepared by membrane ...

We purified the p19 proteins from the Prague C strain of Rous sarcoma virus, avian myeloblastosis virus, B77 sarcoma virus, myeloblastosis-associated virus-2(0), and PR-E 95-C virus and measured their binding affinities for 60S viral RNA by the nitrocellulose filter binding technique. The apparent association constants of the p19 proteins from Rous sarcoma virus Prague C, avian myeloblastosis v...

The golden mussel Limnoperna fortunei (Dunker, 1857) (Mytilidae) is an invading species in continental waters in the Neotropical Region. L. fortunei is transported by human activities. Differing from fresh water bivalves native to the region, this mytilid has external fertility and a planktonic larval development similar to marine mytilids (Monteiros-Ribas et al., 2006). The impact produced by ...

Infectious Epstein-Barr virus (EBV) was produced from suspension cultures of P3HR-1 cells. A protocol for the scaled-up production and concentration of the virus was developed. Virus from the culture fluid was concentrated by continuous-flow pelletization or continuous flow with banding in sucrose. EBV prepared by pelletization yielded 1.7 X 10(4) infectious units/ml (100 X concentration) and l...

hemophagocytic lymphohistiocytosis (hlh) is one of the complications of epstein-barr virus (ebv) infection. although the patients who have developed hlh following ebv have normal immune system, there are a few patients with ebv-induced immune deficiency who develop hlh as well. here, we describe the case of a 10-year-old girl with neurological complications caused by ebv-induced hlh. the patien...