The enzyme amylase was shown to be present in extracts prepared from both house dust and spent growth medium used in the culture of the mite Dermatophagoides pteronyssinus. In dust, it was shown to correlate with both mite counts and concentrations of the faecally derived mite allergen, Der p I. Mite amylase was isolated from the culture medium and shown to be a single chain protein with a mole...

Gamma-interferon-inducible lysosomal thiol reductase (GILT) is known to reduce disulfide bonds present in proteins internalized by antigen presenting cells, facilitating optimal processing and presentation of peptides on Major Histocompatibility Complex class II molecules, as well as the subsequent activation of CD4-positive T lymphocytes. Here, we show that GILT is required for class II-restri...

atopic dermatitis (ad) is a heterogeneous disease with regard to clinical phenotype and natural history. we investigated t cell subtypes and cytokine responses in peripheral blood and skin lesions of ad patients with various sensitivities. immunological studies were performed in 27 subjects: 9 house dust mite (hdm)-sensitized; 6 subjects with sensitizations other than hdm; 7 non-allergic ad pat...

This study was concerned with the correlation between skin test, bronchial provocation test, and histamine release from leucocytes and the serum level of total and allergen-specific IgE in patients atopic to house-dust mite allergen (Dermatophagiodes pteronyssinus) as measured by an intracutaneous skin test. It is concluded that, assuming a positive provocation test to be evidence of allergy, e...

BACKGROUND The relationship between mite and pet allergen exposure in infancy and the subsequent development of sensitization and asthma is complex. OBJECTIVE We prospectively investigated the effect of allergen exposure at 3 months of age on the development of sensitization, wheeze, and physician-diagnosed asthma in the first 4 years of life in a birth cohort of children with and without an ...

house dust mites – stability of the mite aller-gen – history of house dust allergy House dust mites known to be an allergen source for 50 years: Der p 1 still detectable in the original sample To the Editors: A German-language publication that appeared 50 years ago (October 1964) can be considered a milestone in the history of allergology: Reindert Voor-horst and the married couple Frits T. Spi...

Background One of the main strategies to reduce the expression of allergic symptoms in atopic patients sensitized to house dust mites is the use of detection system for monitoring and reduce environmental allergens in indoor spaces. In this context the IgY technology represent a valuable tool. The specific aims of this study was to standardize a in house method of indirect ELISA assay for detec...

This paper describes a current multi-disciplinary UK funded research project aimed at developing a hygrothermal model of house dust mite response to environmental conditions. The project involves the development of a transient hygrothermal bed model, which has been tested and validated by comparing its predictions with the environmental conditions measured in a bed in a test laboratory and in b...

BACKGROUND The aim of the present study was to determine the distribution of mite allergens in pig-farming environments in comparison to urban homes and the relationship between exposure to mite allergens and sensitization to the respective allergens in 100 pig farmers with work-related respiratory symptoms. METHODS The concentration of storage mite (Lep d 2) and house-dust-mite (Der p 1, Der...