Conventional quantity determination of Chinese herbal medicines by High-Performance ThinLayer Chromatography (HPTLC) must be carried out on a TLC scanner for measuring the marker component on the HPTLC plate. Generally the HPTLC image of the herbal drug always takes the photograph as the permanent visual document. Considering the photo of the HPTLC image preserving all the visual quality-relate...

Availability of rapid and reliable methods for detection of quality in plant raw materials and botanicals is urgently needed. The recent HPTLC instrumentation allows to obtain fingerprints useful to ascertain identity and composition. The results of direct application of HPTLC devices in selected cases, using the fingerprint approach, are here reported, considered and compared with other method...

The aim of the work is to develop a simple, rapid, selective and cost effective HPTLC method for the determination of galangin in Alpinia galanga & Alpinia officinarum. The HPTLC densitometric technique was therefore, selected for the quantitative and qualitative determination of galangin in Alpinia galanga and Alpinia officinarum respectively. There are different analytical methods were used t...

Liquid-liquid extraction-thin layer chromatography (LLE-TLC) has been a common and routine combined method for detection of drugs in biological materials. Solid-phase extraction (SPE) is gradually replacing the traditional LLE method. High performance thin layer chromatography (HPTLC) has several advantages over TLC. The present work studied the higher efficiency of a new SPE-HPTLC method over ...

PURPOSE This study evaluates the correlation between corneal densitometry and epithelial ingrowth (EI) after laser in situ keratomileusis (LASIK). METHODS Corneal densitometry of 3 patients who developed EI after LASIK was measured with the Oculus Pentacam. Corneal densitometry readings of each patient were obtained preoperatively and postoperatively after ingrowth was discovered. Densitometr...

High performance thin layer chromatography (HPTLC) combined with on-spot detection and characterization via easy ambient sonic-spray ionization mass spectrometry (EASI-MS) is applied to the analysis of biodiesel (B100) and biodiesel-petrodiesel blends (BX). HPTLC provides chromatographic resolution of major components whereas EASI-MS allows on-spot characterization performed directly on the HPT...

High-performance thin-layer chromatography (HPTLC)-bioassays are promising new methods for detecting bioactive chemicals in food packaging. Here, we test whether direct-acting genotoxic detectable contact materials (FCM) using HPTLC-bioassays. First, an interactive worksheet lays out steps to calculate needed detection limits (bio)analytical from regulatory limits, including thresholds of toxic...

Abstract High-performance thin-layer chromatography (HPTLC), as the modern form of TLC (thin-layer chromatography), is suitable for detecting pharmaceutically active compounds over a wide polarity range using gradient multiple development (GMD) technique. Diode-array detection (DAD) in conjunction with HPTLC can simultaneously acquire ultraviolet?visible (UV?VIS) and fluorescence spectra direct...