The majority of previous work examining stress responses has been done in males. Recently, it has become clear that the impact of stressor exposure is modulated by sex. One stress response that may be affected by sex is the induction of intracellular heat shock protein (HSP) 72, which is a stress- responsive molecular chaperone that refolds denatured proteins and promotes cellular survival. The...

Lee, W. C., H. C. Wen, C. P. Chang, M. Y. Chen, and M. T. Lin. Heat shock protein 72 overexpression protects against hyperthermia, circulatory shock, and cerebral ischemia during heatstroke. J Appl Physiol 100: 2073–2082, 2006; doi:10.1152/japplphysiol.01433.2005.— This study extends our earlier studies in rats by applying our heatstroke model to a new species. Additionally, transgenic mice are...

The nephrotoxicity of gentamicin (GM) has been widely recognized. Heat shock protein 72 (HSP72) has been reported to be a cytoprotectant. However, its cytoprotective effect against GM induced kidney injury has not yet been studied. In this study, we investigated the cytoprotective effect of HSP72 on GM-induced nephrotoxicity in vitro. Human Kidney tubular cell line, HK-2 cells were divided into...

Hsp70 proteins represent a family of chaperones that regulate cellular homeostasis and are required for cancer cell survival. However, their function and regulation in mitosis remain unknown. In this paper, we show that the major inducible cytoplasmic Hsp70 isoform, Hsp72, is required for assembly of a robust bipolar spindle capable of efficient chromosome congression. Mechanistically, Hsp72 as...

To evaluate the cellular response to traumatic brain injury, the expression of mRNA for c-fos and the 72-kDa heat shock protein (hsp72) was determined using in situ hybridization following lateral fluid-percussion injury (2.2-2.4 atm) in rat brain. At 2 h after injury, induction of c-fos mRNA was observed throughout the cortex ipsilateral to the site of injury, while increased expression of hsp...

Nickerson, M., G. F. Elphick, J. Campisi, B. N. Greenwood, and M. Fleshner. Physical activity alters the brain Hsp72 and IL-1 responses to peripheral E. coli challenge. Am J Physiol Regul Integr Comp Physiol 289: R1665–R1674, 2005. First published August 4, 2005; doi:10.1152/ajpregu.00601.2004.—Physically active rats have facilitated heat shock protein 72 (Hsp72) responses after stressor exposu...

Previously, we demonstrated gender differences in Na-K-ATPase (NKA) expression and function after renal ischemia-reperfusion (I/R) injury (Sex differences in the alterations of Na(+), K(+)-ATPase following ischemia-reperfusion injury in the rat kidney. J Physiol 555: 471-480, 2004). Postischemic membrane destruction causes inhibition of NKA, whereas heat shock protein (HSP) 72 helps to preserve...

AIM To investigate the effect of temperature-humidity index (THI) on the expression pattern of heat-shock protein 72 (HSP72) mRNA of Sahiwal and Karan-Fries (KF) cattle in different THIs. MATERIALS AND METHODS Five different periods of a year were selected based on combinations of Tmax/Tmin, viz., P1: <20°C/<10°C; P2: >20°C/<10°C, P3: <30°C/<15°C; P4: >35°C/<20°C, and P5: >35°C/>20°C. The THI...

Induction of heat shock protein (HSP) 72 improves metabolic profiles in diabetic model mice. However, its effect on pancreatic b-cells is not known. The current study investigated whether HSP72 induction can reduce b-cell stress signaling and apoptosis and preserve b-cell mass. MIN6 cells and db/db mice were shamtreated or treated with heat shock (HS) and mild electrical stimulation (MES) (HS+M...