Counting the different subpopulations of cells in a fingerprick of human blood is important for a number of clinical point-of-care (PoC) applications. It is a challenge to demonstrate the integration of sample preparation and detection techniques in a single platform. In this paper we demonstrate a generic microfluidic platform that combines sample processing and characterisation and enumeratio...

Counting the different subpopulations of cells in a fingerprick of human blood is important for a number of clinical point-of-care (PoC) applications. It is a challenge to demonstrate the integration of sample preparation and detection techniques in a single platform. In this paper we demonstrate a generic microfluidic platform that combines sample processing and characterisation and enumeratio...

In this paper, we review the adaptive design methodology of Li et al. (Biostatistics 3:277-287) for two-stage trials with mid-trial sample size adjustment. We argue that it is closer in principle to a group sequential design, in spite of its obvious adaptive element. Several extensions are proposed that aim to make it even more attractive and transparent alternative to a standard (fixed sample ...

Blood cells extraction and detection is very much important for all human beings, because there are WBCs, RBCs, Platelets in our blood, White blood cell count gives the vital information that help diagnosis many of the patient’s sickness. This work presents a new adaptive approach of extracting and detecting the WBCs in blood sample microscopic images. In the research work the author used two d...

Most inaccuracies in the analysis of gases and electrolytes in arterial blood samples are due to preanalytic factors, among which is the type of equipment used for blood collection. Our objective was to compare arterial blood gas sample kits used under clinical conditions and to evaluate the impact of delay in estimation on variability in results. In 2 types of study we compared 5 kits (Radiome...

INTRODUCTION Homogeneity of sample achieved by adequate mixing of the blood sample is the key to the quality of analytical results. Immediate mixing of the sample upon blood collection insures that the anticoagulant in the specimen container has fully dissolved with the blood, thus maintaining not only the effective anticoagulant-to-blood ratio but also providing a specimen free from any blood ...

To simplify our procedure for blood sample processing for PCR, we introduced a simpler, shorter, and more cost-effective method for the separation of peripheral blood lymphocytes (PBL) and DNA extraction for amplification with Taq polymerase. By this method, blood samples are processed in two simple 15-min steps: (1) separation of PBLs from whole blood by red blood cell lysis with the Roche Spe...